Expression of MTAI promotes motility and invasiveness of PANC-I pancreatic carcinoma cells

M. D. Hofer, A. Menke, F. Genze, P. Gierschik, K. Giehl*

*Corresponding author for this work

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

The human metastasis-associated protein I (MTAI) is a constituent of the nucleosome-remodelling and -deacetylation complex. Its expression has been correlated with the invasion and metastasis of epithelial neoplasms. To address the functional consequences of MTAI expression in pancreatic carcinoma cells, we have established PANC-I pancreatic carcinoma cells that stably express MTAI as an enhanced green fluorescent fusion protein (EGFP-MTAI). Here, we demonstrate that heterologous expression of EGFP-MTAI markedly enhanced the cellular motility and the invasive penetration of epithelial barriers by the cells. Expression of EGFP-MTAI had no effect on substrate-independent growth, but reduced substrate-dependent cell proliferation. In addition, the organisation of the cytokeratin filament system and the localisation of the actin cytoskeleton-associated protein IQGAPI were distinctly altered in EGFP-MTAI-expressing cells. These results indicate that enhanced expression of MTAI promotes the acquisition of an invasive, metastatic phenotype, and thus enhances the malignancy of pancreatic adenocarcinoma cells by modulation of the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalBritish Journal of Cancer
Volume90
Issue number2
DOIs
StatePublished - Jan 26 2004

Keywords

  • Invasion and metastasis
  • MTAI
  • Migration
  • Pancreatic carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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