Expression of peroxisomal enoyl-coa hydratase/3-hydroxyacyl-coa dehydrogenase enzyme and its mRNA in peroxisome proliferator-induced liver tumors

M. Sambasiva Rao*, Hideyuki Ide, Anjana V. Yeldandi, Sujata Kumar, Janardan K. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have examined ciprofibrate and dehydroepiandrosterone (DHEA)-induced hepatic lesions for the peroxisomal β-oxidation system enzyme peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (PBE) and its mRNA using SDS-polyacrylamide gel electrophoresis, antibodies and cDNA probe. All 12 neoplastic nodules and nine hepatocellular carcinomas (HCCs) that were analyzed for PBE mRNA by in situ hybridization showed an intense signal comparable to the adjacent non-neoplastic liver. SDS-polyacrylamide gel electrophoresis of postnuclear fractions of six HCC and adjacent liver tissue showed a marked increase in an 80 kDa polypeptide. Immunoblot and Northern blot analysis showed a marked increase in PBE enzyme and PBE mRNA respectively in HCC and adjacent non-neoplastic liver tissue. In control livers (animals not treated with peroxisome proliferators), the levels of PBE enzyme and mRNA were very low or undetectable. The results of this study clearly indicate that peroxisomal proliferator (PP)-induced liver lesions express peroxisomal enzymes to the same extent as adjacent liver and that these enzymes are not useful markers for identification of PP-induced lesions. Oxford University Press.

Original languageEnglish (US)
Pages (from-to)2619-2622
Number of pages4
JournalCarcinogenesis
Volume15
Issue number11
DOIs
StatePublished - Nov 1994

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'Expression of peroxisomal enoyl-coa hydratase/3-hydroxyacyl-coa dehydrogenase enzyme and its mRNA in peroxisome proliferator-induced liver tumors'. Together they form a unique fingerprint.

Cite this