Expression of S-100 protein in renal cell neoplasms

Fan Lin; Wannian Yang; Mark Betten; Bin Tean Teh; Ximing J. Yang; Stephane Richard; Sophie Giraud; Sophie Ferlicot; Philippe Vielh; Delphine Amsellem-Ouazana; Bernard Debré; Thierry Flam; Nicolas Thiounn; Marc Zerbib; Gérard Benoît; Stéphane Droupy; Vincent Molinié; Annick Vieillefond

doi:10.1016/j.humpath.2005.12.008

Expression of S-100 protein in renal cell neoplasms

Fan Lin^*, Wannian Yang, Mark Betten, Bin Tean Teh, Ximing J. Yang, Stephane Richard, Sophie Giraud, Sophie Ferlicot, Philippe Vielh, Delphine Amsellem-Ouazana, Bernard Debré, Thierry Flam, Nicolas Thiounn, Marc Zerbib, Gérard Benoît, Stéphane Droupy, Vincent Molinié, Annick Vieillefond

^*Corresponding author for this work

Pathology

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Abstract

Polyclonal antibody to S-100 protein has been routinely applied for initial screening of various types of tumors, including, melanocytic tumors and neurogenic tumors. S-100 protein has been shown to have a broad distribution in human tissues, including renal tubules. The potential utility of S-100 protein in renal cell neoplasms has not been extensively investigated. Using an EnVision-Horseradish Peroxidase (HRP; Dako, Carpinteria, Calif) kit, we evaluated the diagnostic value of S-100 protein on tissue microarray sections from 175 cases of renal epithelial neoplasm (145 primary renal neoplasms and 30 metastatic renal cell carcinomas) and 24 non-neoplastic renal tissues. Immunohistochemical stains for pancytokeratin, HMB-45, and Mart-1 were also performed. Western blot using the same antibody (anti-S-100 protein) was performed on 10 cases of renal cell neoplasm. The results demonstrated that nuclear and cytoplasmic staining pattern for S-100 protein was observed in 56 (69%) of 81 conventional (clear cell) renal cell carcinomas (RCCs), 10 (30%) of 33 papillary RCCs, 1 (6%) of 16 ChRCCs, and 13 (87%) of 15 oncocytomas. Among the 81 cases of CRCC, positivity for S-100 protein was seen in 41 (71%) of 58 and 15 (65%) of 23 cases with Furhman nuclear grade I/II and III/IV, respectively. Focal immunostaining was present in 22 (92%) of 24 normal renal tubules. Similar staining pattern was observed in 21 (70%) of 30 metastatic RCCs. Western blotting demonstrated the S-100 protein expression in both renal cell neoplasm and normal renal tissue. Overexpression of S-100 in oncocytomas compared with ChRCCs was confirmed by the data of Western blot and cDNA microarray analysis. Importantly, 14.8% (12/81) of clear cell RCC and 13.3% (4/30) of metastatic RCC revealed an immunostaining profile of pancytokeratin (-)/S-100 protein (+). These data indicate that caution should be taken in interpreting an unknown primary with S-100 positivity and cytokeratin negativity. In addition, it suggests that S-100 has a diagnostic value in differentiating oncocytoma from RCC.

Original language	English (US)
Pages (from-to)	462-470
Number of pages	9
Journal	Human pathology
Volume	37
Issue number	4
DOIs	https://doi.org/10.1016/j.humpath.2005.12.008
State	Published - Apr 2006

Keywords

Chromophobe renal cell carcinoma
Metastatic renal cell carcinoma
Oncocytoma
Pancytokeratin
Primary renal cell carcinoma
S-100 protein

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/j.humpath.2005.12.008

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Lin, F., Yang, W., Betten, M., Teh, B. T., Yang, X. J., Richard, S., Giraud, S., Ferlicot, S., Vielh, P., Amsellem-Ouazana, D., Debré, B., Flam, T., Thiounn, N., Zerbib, M., Benoît, G., Droupy, S., Molinié, V., & Vieillefond, A. (2006). Expression of S-100 protein in renal cell neoplasms. Human pathology, 37(4), 462-470. https://doi.org/10.1016/j.humpath.2005.12.008

@article{e11c2989ab474763aa74a5dafe04a931,

title = "Expression of S-100 protein in renal cell neoplasms",

abstract = "Polyclonal antibody to S-100 protein has been routinely applied for initial screening of various types of tumors, including, melanocytic tumors and neurogenic tumors. S-100 protein has been shown to have a broad distribution in human tissues, including renal tubules. The potential utility of S-100 protein in renal cell neoplasms has not been extensively investigated. Using an EnVision-Horseradish Peroxidase (HRP; Dako, Carpinteria, Calif) kit, we evaluated the diagnostic value of S-100 protein on tissue microarray sections from 175 cases of renal epithelial neoplasm (145 primary renal neoplasms and 30 metastatic renal cell carcinomas) and 24 non-neoplastic renal tissues. Immunohistochemical stains for pancytokeratin, HMB-45, and Mart-1 were also performed. Western blot using the same antibody (anti-S-100 protein) was performed on 10 cases of renal cell neoplasm. The results demonstrated that nuclear and cytoplasmic staining pattern for S-100 protein was observed in 56 (69%) of 81 conventional (clear cell) renal cell carcinomas (RCCs), 10 (30%) of 33 papillary RCCs, 1 (6%) of 16 ChRCCs, and 13 (87%) of 15 oncocytomas. Among the 81 cases of CRCC, positivity for S-100 protein was seen in 41 (71%) of 58 and 15 (65%) of 23 cases with Furhman nuclear grade I/II and III/IV, respectively. Focal immunostaining was present in 22 (92%) of 24 normal renal tubules. Similar staining pattern was observed in 21 (70%) of 30 metastatic RCCs. Western blotting demonstrated the S-100 protein expression in both renal cell neoplasm and normal renal tissue. Overexpression of S-100 in oncocytomas compared with ChRCCs was confirmed by the data of Western blot and cDNA microarray analysis. Importantly, 14.8% (12/81) of clear cell RCC and 13.3% (4/30) of metastatic RCC revealed an immunostaining profile of pancytokeratin (-)/S-100 protein (+). These data indicate that caution should be taken in interpreting an unknown primary with S-100 positivity and cytokeratin negativity. In addition, it suggests that S-100 has a diagnostic value in differentiating oncocytoma from RCC.",

keywords = "Chromophobe renal cell carcinoma, Metastatic renal cell carcinoma, Oncocytoma, Pancytokeratin, Primary renal cell carcinoma, S-100 protein",

author = "Fan Lin and Wannian Yang and Mark Betten and Teh, {Bin Tean} and Yang, {Ximing J.} and Stephane Richard and Sophie Giraud and Sophie Ferlicot and Philippe Vielh and Delphine Amsellem-Ouazana and Bernard Debr{\'e} and Thierry Flam and Nicolas Thiounn and Marc Zerbib and G{\'e}rard Beno{\^i}t and St{\'e}phane Droupy and Vincent Molini{\'e} and Annick Vieillefond",

year = "2006",

month = apr,

doi = "10.1016/j.humpath.2005.12.008",

language = "English (US)",

volume = "37",

pages = "462--470",

journal = "Human Pathology",

issn = "0046-8177",

publisher = "W.B. Saunders Ltd",

number = "4",

}

TY - JOUR

T1 - Expression of S-100 protein in renal cell neoplasms

AU - Lin, Fan

AU - Yang, Wannian

AU - Betten, Mark

AU - Teh, Bin Tean

AU - Yang, Ximing J.

AU - Richard, Stephane

AU - Giraud, Sophie

AU - Ferlicot, Sophie

AU - Vielh, Philippe

AU - Amsellem-Ouazana, Delphine

AU - Debré, Bernard

AU - Flam, Thierry

AU - Thiounn, Nicolas

AU - Zerbib, Marc

AU - Benoît, Gérard

AU - Droupy, Stéphane

AU - Molinié, Vincent

AU - Vieillefond, Annick

PY - 2006/4

Y1 - 2006/4

N2 - Polyclonal antibody to S-100 protein has been routinely applied for initial screening of various types of tumors, including, melanocytic tumors and neurogenic tumors. S-100 protein has been shown to have a broad distribution in human tissues, including renal tubules. The potential utility of S-100 protein in renal cell neoplasms has not been extensively investigated. Using an EnVision-Horseradish Peroxidase (HRP; Dako, Carpinteria, Calif) kit, we evaluated the diagnostic value of S-100 protein on tissue microarray sections from 175 cases of renal epithelial neoplasm (145 primary renal neoplasms and 30 metastatic renal cell carcinomas) and 24 non-neoplastic renal tissues. Immunohistochemical stains for pancytokeratin, HMB-45, and Mart-1 were also performed. Western blot using the same antibody (anti-S-100 protein) was performed on 10 cases of renal cell neoplasm. The results demonstrated that nuclear and cytoplasmic staining pattern for S-100 protein was observed in 56 (69%) of 81 conventional (clear cell) renal cell carcinomas (RCCs), 10 (30%) of 33 papillary RCCs, 1 (6%) of 16 ChRCCs, and 13 (87%) of 15 oncocytomas. Among the 81 cases of CRCC, positivity for S-100 protein was seen in 41 (71%) of 58 and 15 (65%) of 23 cases with Furhman nuclear grade I/II and III/IV, respectively. Focal immunostaining was present in 22 (92%) of 24 normal renal tubules. Similar staining pattern was observed in 21 (70%) of 30 metastatic RCCs. Western blotting demonstrated the S-100 protein expression in both renal cell neoplasm and normal renal tissue. Overexpression of S-100 in oncocytomas compared with ChRCCs was confirmed by the data of Western blot and cDNA microarray analysis. Importantly, 14.8% (12/81) of clear cell RCC and 13.3% (4/30) of metastatic RCC revealed an immunostaining profile of pancytokeratin (-)/S-100 protein (+). These data indicate that caution should be taken in interpreting an unknown primary with S-100 positivity and cytokeratin negativity. In addition, it suggests that S-100 has a diagnostic value in differentiating oncocytoma from RCC.

AB - Polyclonal antibody to S-100 protein has been routinely applied for initial screening of various types of tumors, including, melanocytic tumors and neurogenic tumors. S-100 protein has been shown to have a broad distribution in human tissues, including renal tubules. The potential utility of S-100 protein in renal cell neoplasms has not been extensively investigated. Using an EnVision-Horseradish Peroxidase (HRP; Dako, Carpinteria, Calif) kit, we evaluated the diagnostic value of S-100 protein on tissue microarray sections from 175 cases of renal epithelial neoplasm (145 primary renal neoplasms and 30 metastatic renal cell carcinomas) and 24 non-neoplastic renal tissues. Immunohistochemical stains for pancytokeratin, HMB-45, and Mart-1 were also performed. Western blot using the same antibody (anti-S-100 protein) was performed on 10 cases of renal cell neoplasm. The results demonstrated that nuclear and cytoplasmic staining pattern for S-100 protein was observed in 56 (69%) of 81 conventional (clear cell) renal cell carcinomas (RCCs), 10 (30%) of 33 papillary RCCs, 1 (6%) of 16 ChRCCs, and 13 (87%) of 15 oncocytomas. Among the 81 cases of CRCC, positivity for S-100 protein was seen in 41 (71%) of 58 and 15 (65%) of 23 cases with Furhman nuclear grade I/II and III/IV, respectively. Focal immunostaining was present in 22 (92%) of 24 normal renal tubules. Similar staining pattern was observed in 21 (70%) of 30 metastatic RCCs. Western blotting demonstrated the S-100 protein expression in both renal cell neoplasm and normal renal tissue. Overexpression of S-100 in oncocytomas compared with ChRCCs was confirmed by the data of Western blot and cDNA microarray analysis. Importantly, 14.8% (12/81) of clear cell RCC and 13.3% (4/30) of metastatic RCC revealed an immunostaining profile of pancytokeratin (-)/S-100 protein (+). These data indicate that caution should be taken in interpreting an unknown primary with S-100 positivity and cytokeratin negativity. In addition, it suggests that S-100 has a diagnostic value in differentiating oncocytoma from RCC.

KW - Chromophobe renal cell carcinoma

KW - Metastatic renal cell carcinoma

KW - Oncocytoma

KW - Pancytokeratin

KW - Primary renal cell carcinoma

KW - S-100 protein

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ER -

Expression of S-100 protein in renal cell neoplasms

Abstract

Keywords

ASJC Scopus subject areas

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