Expression of SET, an inhibitor of protein phosphatase 2A, in renal development and Wilms' tumor

Sara G. Carlson, Eudora Eng, Eung Gook Kim, Elizabeth J. Peruvian, Terry D. Copel, Barbara J. Ballermann*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

The human gene set was originally identified as a component of the set- can fusion gene produced by a somatic translocation event in a case of acute undifferentiated leukemia. In the developing kidney, set was highly expressed in the zone of nephron morphogenesis. Recently, SET was shown to be a potent and specific inhibitor of protein phosphatase 2A, a family of major serine/threonine phosphatases involved in regulating cell proliferation and differentiation. The current study sought to define further the role of SET in the regulation of renal cell proliferation and tumorigenesis. The mRNA encoding SET was expressed at much higher levels in transformed human and rodent cell lines than in cultured renal epithelial and primary endothelial cells. Consistent with a role for SET in cell proliferation, set mRNA expression was markedly reduced in cells rendered quiescent by serum starvation, contact inhibition, or differentiation. Previous findings during renal development were extended by demonstrating that SET protein expression is also much greater in developing rat and human kidney than in fully differentiated, mature kidney. Finally, high levels of set mRNA and SET protein expression were found in Wilms' tumor, but not in renal cell carcinoma, adult polycystic kidney disease or in transitional cell carcinoma.

Original languageEnglish (US)
Pages (from-to)1873-1880
Number of pages8
JournalJournal of the American Society of Nephrology
Volume9
Issue number10
StatePublished - 1998

ASJC Scopus subject areas

  • Nephrology

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