Expression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression/secretion vector in Saccharomyces cerevisiae

Jolanta Topczewska, Bozenna Rempoła, Magdalena Fikus*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture conditions. Both cloned genes code for biotechnologically important proteins: human epidermal growth factor and a serine proteinase inhibitor from Cucurbitacea.

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalActa biochimica Polonica
Volume43
Issue number1
DOIs
StatePublished - 1996

Keywords

  • Cloning in Saccharomyces cerevisiae
  • Human epidermal growth factor
  • Serine proteinase inhibitor
  • Yeast expression/secretion vector

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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