TY - JOUR
T1 - Expression of the β1 and β2(AMOG) subunits of the Na,K-ATPase in neural tissues
T2 - Cellular and developmental distribution patterns
AU - Lecuona, Emilia
AU - Luquín, Sonia
AU - Avila, Julio
AU - García-Segura, Luis M.
AU - Martín-Vasallo, Pablo
N1 - Funding Information:
We thank Dr. Julie Chowen (Cajal Institute) for invaluable editorial help of the manuscript and Dr. Kathleen Sweadner (Harvard University) for critical reading of the manuscript. This work was supported by grant 93/0831 from Fondo de Investigaciones Sanitarias de la Seguridad Social (FIS) (Spain), to P. M.-V. and grant DGICYT PM92-0022 to L.M.G.-S. S.L. Is supported by a fellowship from the International Co-operation Treaty of CONACYT (Mexico) and C.S.I.C (Spain).
PY - 1996
Y1 - 1996
N2 - We have used isoform-specific antisera against the Na,K-ATPase β1 (SpETb1) and β2(AMOG) (SpETb2) subunit isoforms in order to establish their specific cellular and subcellular localization in several developmental stages of the rat central nervous system. Immunocytochemical preparations revealed β1 isoform protein in most neural cells, being predominantly located in the soma of neurons and astrocytes, with no appreciable developmental variations. In the newborn rat, β2(AMOG) immunoreactivity was present in cellular processes of astroglia and in the somas of neurons and decreasing in intensity with maturation until adulthood, where no β2 isoform was detected in neurons. The differential location of these isoforms, both developmentally and at the cellular level suggest a complex regulation of their genes expression and mechanisms of subcellular distribution, as well as functional differences.
AB - We have used isoform-specific antisera against the Na,K-ATPase β1 (SpETb1) and β2(AMOG) (SpETb2) subunit isoforms in order to establish their specific cellular and subcellular localization in several developmental stages of the rat central nervous system. Immunocytochemical preparations revealed β1 isoform protein in most neural cells, being predominantly located in the soma of neurons and astrocytes, with no appreciable developmental variations. In the newborn rat, β2(AMOG) immunoreactivity was present in cellular processes of astroglia and in the somas of neurons and decreasing in intensity with maturation until adulthood, where no β2 isoform was detected in neurons. The differential location of these isoforms, both developmentally and at the cellular level suggest a complex regulation of their genes expression and mechanisms of subcellular distribution, as well as functional differences.
KW - AMOG
KW - Adhesion molecule on glia
KW - Na,K-ATPase β subunit isoforms
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U2 - 10.1016/0361-9230(96)00042-1
DO - 10.1016/0361-9230(96)00042-1
M3 - Article
C2 - 8736577
AN - SCOPUS:0030011114
SN - 0361-9230
VL - 40
SP - 167
EP - 174
JO - Brain Research Bulletin
JF - Brain Research Bulletin
IS - 3
ER -