Expression of the apoptosis-related genes, caspase-1, caspase-3, DNA fragmentation factor, and apoptotic protease activating factor-1, in human granulosa cells

Masao Izawa, Phuong H. Nguyen, Helen Kim, John Yeh*

*Corresponding author for this work

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Objective: To investigate protein and messenger RNA expression products for a subset of apoptosis-related genes in human granulosa cells. Design: In vitro experiment. Setting: Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts. Patient(s): Women undergoing oocyte retrieval for IVF after ovulation induction with gonadotropins. Intervention(s): Granulosa cells were isolated from follicular aspirates after oocyte removal. Main Outcome Measure(s): Reverse transcription polymerase chain reaction and Western blotting for apoptosis-related gene products. Result(s): The expression of caspase-1, caspase-3, DNA fragmentation factor, and apoptotic protease activating factor-1, which are intermediate molecules in phylogenetically conserved apoptotic pathways, was demonstrated in granulosa cells from patients undergoing IVF. Moreover, proforms, but not activated enzymes, for both caspase-1 and caspase-3 were observed. Conclusion(s): Granulosa cells from patients undergoing IVF have intrinsic apoptotic machinery that could be activated for tissue remodeling.

Original languageEnglish (US)
Pages (from-to)549-552
Number of pages4
JournalFertility and Sterility
Volume70
Issue number3
DOIs
StatePublished - Oct 5 1998

Keywords

  • Apoptosis
  • Apoptotic machinery
  • Apoptotic protease activating factor-1
  • Caspases
  • DNA fragmentation factor
  • Granulosa cells
  • Human ovary

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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