Expression of the c-Ha-ras and c-mye genes in aflatoxin B1-induced hepatocellular carcinomas

Fumio Tashiro; Shigeru Morimura; Kenshi Hayashi; Reiko Makino; Hideki Kawamura; Nobuo Horikoshi; Kiyomitsu Nemoto; Koichiro Ohtsubo; Takashi Sugimura; Yoshio Ueno

doi:10.1016/S0006-291X(86)80575-7

Expression of the c-Ha-ras and c-mye genes in aflatoxin B₁-induced hepatocellular carcinomas

Fumio Tashiro^*, Shigeru Morimura, Kenshi Hayashi, Reiko Makino, Hideki Kawamura, Nobuo Horikoshi, Kiyomitsu Nemoto, Koichiro Ohtsubo, Takashi Sugimura, Yoshio Ueno

^*Corresponding author for this work

Radiation Oncology

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Abstract

Expression and activation of several c-oncogenes in seven hepatocellular carcinomas from seven separate rats treated with aflatoxin B₁ (AFB₁) were examined by Northern and Southern blot analyses. Both c-Ha-ras and c-myc transcripts were elevated at high levels in all hepatomas. Moreover, in one of them, T2-1 hepatoma, the c-myc gene was amplified only in a tumor part of liver without significant rearrangement. N-ras specific transcripts were not elevated in these hepatomas. The present data suggest that the consistently increased expression or deregulation of the c-myc and c-Ha-ras genes may play an important role in the development of hepatomas induced by AFB₁.

Original language	English (US)
Pages (from-to)	858-864
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	138
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80575-7
State	Published - Jul 31 1986

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{cb8484adb5944efab122f33722cd63d6,

title = "Expression of the c-Ha-ras and c-mye genes in aflatoxin B1-induced hepatocellular carcinomas",

abstract = "Expression and activation of several c-oncogenes in seven hepatocellular carcinomas from seven separate rats treated with aflatoxin B1 (AFB1) were examined by Northern and Southern blot analyses. Both c-Ha-ras and c-myc transcripts were elevated at high levels in all hepatomas. Moreover, in one of them, T2-1 hepatoma, the c-myc gene was amplified only in a tumor part of liver without significant rearrangement. N-ras specific transcripts were not elevated in these hepatomas. The present data suggest that the consistently increased expression or deregulation of the c-myc and c-Ha-ras genes may play an important role in the development of hepatomas induced by AFB1.",

author = "Fumio Tashiro and Shigeru Morimura and Kenshi Hayashi and Reiko Makino and Hideki Kawamura and Nobuo Horikoshi and Kiyomitsu Nemoto and Koichiro Ohtsubo and Takashi Sugimura and Yoshio Ueno",

note = "Funding Information: We thank Dr. R.W. Ellis for oroviding clone BS9, Dr. M. Wigler for p6al, Dr. I.M. Verma for pcf_os(human)-i and Dr. S.M. Tilghman for prAlbl. This work was supported in part by Grants-in-Aid for Cancer Research and Biotoxin from the Ministry of Education, Science and Culture of Japan.",

year = "1986",

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doi = "10.1016/S0006-291X(86)80575-7",

language = "English (US)",

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T1 - Expression of the c-Ha-ras and c-mye genes in aflatoxin B1-induced hepatocellular carcinomas

AU - Tashiro, Fumio

AU - Morimura, Shigeru

AU - Hayashi, Kenshi

AU - Makino, Reiko

AU - Kawamura, Hideki

AU - Horikoshi, Nobuo

AU - Nemoto, Kiyomitsu

AU - Ohtsubo, Koichiro

AU - Sugimura, Takashi

AU - Ueno, Yoshio

N1 - Funding Information: We thank Dr. R.W. Ellis for oroviding clone BS9, Dr. M. Wigler for p6al, Dr. I.M. Verma for pcf_os(human)-i and Dr. S.M. Tilghman for prAlbl. This work was supported in part by Grants-in-Aid for Cancer Research and Biotoxin from the Ministry of Education, Science and Culture of Japan.

PY - 1986/7/31

Y1 - 1986/7/31

N2 - Expression and activation of several c-oncogenes in seven hepatocellular carcinomas from seven separate rats treated with aflatoxin B1 (AFB1) were examined by Northern and Southern blot analyses. Both c-Ha-ras and c-myc transcripts were elevated at high levels in all hepatomas. Moreover, in one of them, T2-1 hepatoma, the c-myc gene was amplified only in a tumor part of liver without significant rearrangement. N-ras specific transcripts were not elevated in these hepatomas. The present data suggest that the consistently increased expression or deregulation of the c-myc and c-Ha-ras genes may play an important role in the development of hepatomas induced by AFB1.

AB - Expression and activation of several c-oncogenes in seven hepatocellular carcinomas from seven separate rats treated with aflatoxin B1 (AFB1) were examined by Northern and Southern blot analyses. Both c-Ha-ras and c-myc transcripts were elevated at high levels in all hepatomas. Moreover, in one of them, T2-1 hepatoma, the c-myc gene was amplified only in a tumor part of liver without significant rearrangement. N-ras specific transcripts were not elevated in these hepatomas. The present data suggest that the consistently increased expression or deregulation of the c-myc and c-Ha-ras genes may play an important role in the development of hepatomas induced by AFB1.

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JO - Biochemical and Biophysical Research Communications

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Expression of the c-Ha-ras and c-mye genes in aflatoxin B₁-induced hepatocellular carcinomas

Abstract

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