Expression of the CD40 ligand in t lymphocytes and induction of IgE isotype switching

Ramsay Fuleihan*, Deborah Ahem, Raif S. Geha

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CD40 ligand (CD40L) delivers a contact-dcpendent signal to B cells which, in the presence of interleukin (IL)-4, drives immunoglobulin isotype switching to IgE. CD40L expression in T cells is transient, requires activation of protein kinase C and a rise in intracellular calcium concentration ([Ca2), and is inhibited by cyclosporin A (CsA). CsA also inhibited T-cell-dependent IL-4-driven IgE synthesis. We have found that expression of CD40L is developmentally regulated. Expression ofCD40L was restricted to mature single-positive thymocytes which, in the presence of IL-4, were capable of inducing B cells to undergo IgE isotype switching. CD40L expression was severely decreased in cord blood lymphocytes and was associated with a severely decreased ability to undergo T-celldependent IgE isotype switching. Caremark and from NMC Homecare. RF is the recipient of a Clinical Investigator Award from the NIAID, K08 AI-01091.

Original languageEnglish (US)
Pages (from-to)43-44
Number of pages2
JournalInternational archives of allergy and immunology
Volume107
Issue number1-3
DOIs
StatePublished - Jan 1 1995

Fingerprint

Immunoglobulin Class Switching
CD40 Ligand
Immunoglobulin E
Interleukin-4
Lymphocytes
Cyclosporine
B-Lymphocytes
National Institute of Allergy and Infectious Diseases (U.S.)
T-Lymphocytes
Immunoglobulin Isotypes
Thymocytes
Fetal Blood
Protein Kinase C
Research Personnel
Calcium

Keywords

  • B cells
  • CD40 ligand
  • Cord blood
  • Cyclosporin A
  • IgE
  • Immunoglobulin isotype switching
  • Interleukin-4
  • Newborn
  • T cells
  • Thymocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

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title = "Expression of the CD40 ligand in t lymphocytes and induction of IgE isotype switching",
abstract = "CD40 ligand (CD40L) delivers a contact-dcpendent signal to B cells which, in the presence of interleukin (IL)-4, drives immunoglobulin isotype switching to IgE. CD40L expression in T cells is transient, requires activation of protein kinase C and a rise in intracellular calcium concentration ([Ca2), and is inhibited by cyclosporin A (CsA). CsA also inhibited T-cell-dependent IL-4-driven IgE synthesis. We have found that expression of CD40L is developmentally regulated. Expression ofCD40L was restricted to mature single-positive thymocytes which, in the presence of IL-4, were capable of inducing B cells to undergo IgE isotype switching. CD40L expression was severely decreased in cord blood lymphocytes and was associated with a severely decreased ability to undergo T-celldependent IgE isotype switching. Caremark and from NMC Homecare. RF is the recipient of a Clinical Investigator Award from the NIAID, K08 AI-01091.",
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Expression of the CD40 ligand in t lymphocytes and induction of IgE isotype switching. / Fuleihan, Ramsay; Ahem, Deborah; Geha, Raif S.

In: International archives of allergy and immunology, Vol. 107, No. 1-3, 01.01.1995, p. 43-44.

Research output: Contribution to journalArticle

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AU - Ahem, Deborah

AU - Geha, Raif S.

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AB - CD40 ligand (CD40L) delivers a contact-dcpendent signal to B cells which, in the presence of interleukin (IL)-4, drives immunoglobulin isotype switching to IgE. CD40L expression in T cells is transient, requires activation of protein kinase C and a rise in intracellular calcium concentration ([Ca2), and is inhibited by cyclosporin A (CsA). CsA also inhibited T-cell-dependent IL-4-driven IgE synthesis. We have found that expression of CD40L is developmentally regulated. Expression ofCD40L was restricted to mature single-positive thymocytes which, in the presence of IL-4, were capable of inducing B cells to undergo IgE isotype switching. CD40L expression was severely decreased in cord blood lymphocytes and was associated with a severely decreased ability to undergo T-celldependent IgE isotype switching. Caremark and from NMC Homecare. RF is the recipient of a Clinical Investigator Award from the NIAID, K08 AI-01091.

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