Expression of the neu oncogene under the transcriptional control of the myelin basic protein gene in transgenic mice: Generation of transformed glial cells

C. Hayes, D. Kelly, S. Murayama, A. Komiyama, K. Suzuki, B. Popko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

We have taken a transgenic approach in an effort to specifically transform oligodendrocytes, the myelinating glial cells of the central nervous system (CNS). Transgenic mice were generated with a DNA construct that contained the activated neu oncogene under the transcriptional control of the myelin basic protein (MBP) gene. The MBP/c‐neu transgenic animals have experienced a low incidence of brain tumors that express molecular markers specific to oligodendrocytes, providing a mouse model to study the formation and progression of oligodendrocyte tumors. A tumor from a transgenic animal has been dispersed in culture, and transformed cells that express properties of oligodendrocytes and astrocytes have been maintained. The degree to which these cells express phenotypic characteristics of oligodendrocytes or astrocytes is influenced by culture conditions. These transformed cells should serve as a valuable resource with which to study various molecular and biochemical aspects of the myelination process, as well as the lineage interrelationship of CNS glial cells.

Original languageEnglish (US)
Pages (from-to)175-187
Number of pages13
JournalJournal of Neuroscience Research
Volume31
Issue number1
DOIs
StatePublished - Jan 1992

Keywords

  • myelin protein gene expression
  • oligodendrogliomas
  • transformed oligodendrocytes

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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