Expression of the protein tyrosine phosphatase β2 gene in mouse erythroleukemia cells induces terminal erythroid differentiation

Tsutomu Kume, Toshio Watanabe, Reiko Sanokawo, Dai Chida, Takeshi Nakamura, Michio Oishi*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We have cloned cDNA for protein tyrosine phosphatase β2, which had been implicated in erythroid differentiation of mouse erythroleukemia cells. Expression of cDNA constructs, in which β2 cDNA is placed under the control of mouse metallothionein-I promoter, by ZnCl2 converted a significant portion (20 to 38%) of the cells to erythroid-like cells, which is 25-50% of the erythroid differentiation efficiency observed by conventional erythroid- inducing agents. Furthermore, introduction and expression of altered protein tyrosine phosphatase β2 cDNA constructs designed to produce the enzyme lacking the phosphatase activity inhibited erythroid differentiation by 100- 20%, depending upon the concentration of erythroid-inducing agents employed. These results strongly suggest that protein tyrosine phosphatase β2 is involved in triggering erythroid differentiation in mouse erythroleukemia cells.

Original languageEnglish (US)
Pages (from-to)30916-30921
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number48
DOIs
StatePublished - Dec 14 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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