Expression pattern of transglutaminases in the early differentiation stage of erupting rat incisor

Daniela Caccamo, Debora Di Mauro, Salvatore Condello, Monica Currò, Giuseppa Cutroneo, Giuseppe Pio Anastasi, Riccardo Ientile*, Fabio Trimarchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Several studies demonstrated that transglutaminases play a key role in extracellular matrix stabilization needed for cell differentiation. We evaluated transglutaminase expression and activity in the pre-secretory stage of differentiation of the continuously erupting rat incisor. We observed that transglutaminase-mediated incorporation of monodansylcadaverine into protein substrates was specifically located in the apical loop, and along the basement membrane joining mesenchyme and inner dental epithelium in the odontogenic organ. Enzyme activity was associated with mRNAs for transglutaminase 1 and 2. Notably, labelling cells for these isoenzymes were observed in both mesenchymal and epithelial compartments, but not in the basement membrane, in the ameloblast facing pulp anterior region, where ameloblast and odontoblast differentiation begins. These findings demonstrate that transglutaminase 1 and transglutaminase 2 are expressed at a major extent in the pre-secretory stage of regenerating rat incisor, where they probably play complementary roles in cell signalling between mesenchyme and epithelium and extracellular matrix.

Original languageEnglish (US)
Pages (from-to)49-56
Number of pages8
JournalAmino Acids
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • Cross-linking activity
  • Differentiation
  • Mesenchymal-epithelial interactions
  • Rat incisor
  • Transglutaminase 1
  • Transglutaminase 2

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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