Expression, purification and in vitro activity of apoptin

Guo Jing Sun*, Xin Tong, Yan Dong, Zhi Xian Sun

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A novel protein named apoptin induces a p53-independent, Bcl-2 insensitive type apoptosis in various human tumor cells but not in normal cells. Apoptin is considered to be a promising candidate for safe and effective anti-tumor therapy. Moreover, apoptin may be important for fundamental studies on molecular basis of apoptosis and cancer. Apoptin gene was subcloned into prokaryotic expression vector pPROEXHT and pBV220, respectively. The apoptin protein was obtained from pPROEXHT-apoptin expression system and not from pBV220-apoptin; the former contains a 6 × histidine affinity tag for ease of purification. Expressed protein was purified by chromatography on a co-chelated affinity column and was renatured by dialysis. The renatured apoptin was able to induce purified Hela nuclei apoptosis in vitro even without the participation of the cytoplasm, showing that apoptin expressed in E. coli was active to induce apoptosis.

Original languageEnglish (US)
Pages (from-to)227-228
Number of pages2
JournalActa biochimica et biophysica Sinica
Volume33
Issue number2
StatePublished - Dec 1 2001

Keywords

  • Apoptin
  • Induce apoptosis
  • Prokaryotic expression
  • Purification

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Expression, purification and in vitro activity of apoptin'. Together they form a unique fingerprint.

Cite this