Abstract
We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 995-998 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 42 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 1 2017 |
Funding
National Science Foundation (NSF) (IIP-1068070); National Institutes of Health (NIH) (R01CA182939). The authors thank Lei Tian and Anne Sentenac for helpful discussions. They are also grateful to the Jason Ritt laboratory for supplying brain tissue samples. Professor Bifano acknowledges a financial interest in Boston Micromachines Corporation.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics