Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain*, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, Jerome Mertz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Original languageEnglish (US)
Pages (from-to)995-998
Number of pages4
JournalOptics Letters
Volume42
Issue number5
DOIs
StatePublished - Mar 1 2017

Funding

National Science Foundation (NSF) (IIP-1068070); National Institutes of Health (NIH) (R01CA182939). The authors thank Lei Tian and Anne Sentenac for helpful discussions. They are also grateful to the Jason Ritt laboratory for supplying brain tissue samples. Professor Bifano acknowledges a financial interest in Boston Micromachines Corporation.

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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