Abstract
We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
Original language | English (US) |
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Pages (from-to) | 995-998 |
Number of pages | 4 |
Journal | Optics Letters |
Volume | 42 |
Issue number | 5 |
DOIs | |
State | Published - Mar 1 2017 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics