@article{54caa630e6af4e52bf9e0dff2698cde4,
title = "Extended depth-of-field microscopy with a high-speed deformable mirror",
abstract = "We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.",
author = "Shain, {William J.} and Vickers, {Nicholas A.} and Goldberg, {Bennett B.} and Thomas Bifano and Jerome Mertz",
note = "Funding Information: National Science Foundation (NSF) (IIP-1068070); National Institutes of Health (NIH) (R01CA182939). The authors thank Lei Tian and Anne Sentenac for helpful discussions. They are also grateful to the Jason Ritt laboratory for supplying brain tissue samples. Professor Bifano acknowledges a financial interest in Boston Micromachines Corporation. Publisher Copyright: {\textcopyright} 2017 Optical Society of America.",
year = "2017",
month = mar,
day = "1",
doi = "10.1364/OL.42.000995",
language = "English (US)",
volume = "42",
pages = "995--998",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "5",
}