Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain; Nicholas A. Vickers; Bennett B. Goldberg; Thomas Bifano; Jerome Mertz

doi:10.1364/OL.42.000995

Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain^*, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, Jerome Mertz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Original language	English (US)
Pages (from-to)	995-998
Number of pages	4
Journal	Optics Letters
Volume	42
Issue number	5
DOIs	https://doi.org/10.1364/OL.42.000995
State	Published - Mar 1 2017

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.42.000995

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title = "Extended depth-of-field microscopy with a high-speed deformable mirror",

abstract = "We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.",

author = "Shain, {William J.} and Vickers, {Nicholas A.} and Goldberg, {Bennett B.} and Thomas Bifano and Jerome Mertz",

note = "Funding Information: National Science Foundation (NSF) (IIP-1068070); National Institutes of Health (NIH) (R01CA182939). The authors thank Lei Tian and Anne Sentenac for helpful discussions. They are also grateful to the Jason Ritt laboratory for supplying brain tissue samples. Professor Bifano acknowledges a financial interest in Boston Micromachines Corporation.",

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doi = "10.1364/OL.42.000995",

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T1 - Extended depth-of-field microscopy with a high-speed deformable mirror

AU - Shain, William J.

AU - Vickers, Nicholas A.

AU - Goldberg, Bennett B.

AU - Bifano, Thomas

AU - Mertz, Jerome

N1 - Funding Information: National Science Foundation (NSF) (IIP-1068070); National Institutes of Health (NIH) (R01CA182939). The authors thank Lei Tian and Anne Sentenac for helpful discussions. They are also grateful to the Jason Ritt laboratory for supplying brain tissue samples. Professor Bifano acknowledges a financial interest in Boston Micromachines Corporation.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

AB - We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

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Extended depth-of-field microscopy with a high-speed deformable mirror

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