Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain; Nicholas A. Vickers; Bennett B. Goldberg; Thomas Bifano; Jerome Mertz

doi:10.1364/OL.42.000995

Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain^*, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, Jerome Mertz

^*Corresponding author for this work

Physics and Astronomy

Research output: Contribution to journal › Article

34 Scopus citations

Abstract

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Original language	English (US)
Pages (from-to)	995-998
Number of pages	4
Journal	Optics Letters
Volume	42
Issue number	5
DOIs	https://doi.org/10.1364/OL.42.000995
State	Published - Mar 1 2017

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ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

Cite this

Shain, W. J., Vickers, N. A., Goldberg, B. B., Bifano, T., & Mertz, J. (2017). Extended depth-of-field microscopy with a high-speed deformable mirror. Optics Letters, 42(5), 995-998. https://doi.org/10.1364/OL.42.000995

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10.1364/OL.42.000995