@inproceedings{b3b8728833c8492d800457bbd400de41,
title = "Extended depth-of-field microscopy with a high-speed deformable mirror",
abstract = "Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.",
keywords = "Adaptive Optics, Brain Imaging, Deconvolution, Extended Depth of Field, Fluorescence Microscopy, Neuron Imaging",
author = "Shain, {William J.} and Vickers, {Nicholas A.} and Goldberg, {Bennett B.} and Thomas Bifano and Jerome Mertz",
note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Adaptive Optics and Wavefront Control for Biological Systems III ; Conference date: 28-01-2017 Through 30-01-2017",
year = "2017",
doi = "10.1117/12.2271502",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Joel Kubby and Bifano, {Thomas G.} and Sylvain Gigan",
booktitle = "Adaptive Optics and Wavefront Control for Biological Systems III",
}