Abstract
Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
Original language | English (US) |
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Title of host publication | Adaptive Optics and Wavefront Control for Biological Systems III |
Editors | Joel Kubby, Thomas G. Bifano, Sylvain Gigan |
Publisher | SPIE |
Volume | 10073 |
ISBN (Electronic) | 9781510605879 |
DOIs | |
State | Published - Jan 1 2017 |
Event | Adaptive Optics and Wavefront Control for Biological Systems III - San Francisco, United States Duration: Jan 28 2017 → Jan 30 2017 |
Other
Other | Adaptive Optics and Wavefront Control for Biological Systems III |
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Country | United States |
City | San Francisco |
Period | 1/28/17 → 1/30/17 |
Keywords
- Adaptive Optics
- Brain Imaging
- Deconvolution
- Extended Depth of Field
- Fluorescence Microscopy
- Neuron Imaging
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging