Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain; Nicholas A. Vickers; Bennett B. Goldberg; Thomas Bifano; Jerome Mertz

doi:10.1117/12.2271502

Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain^*, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, Jerome Mertz

^*Corresponding author for this work

Physics and Astronomy

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Original language	English (US)
Title of host publication	Adaptive Optics and Wavefront Control for Biological Systems III
Editors	Joel Kubby, Thomas G. Bifano, Sylvain Gigan
Publisher	SPIE
Volume	10073
ISBN (Electronic)	9781510605879
DOIs	https://doi.org/10.1117/12.2271502
State	Published - Jan 1 2017
Event	Adaptive Optics and Wavefront Control for Biological Systems III - San Francisco, United States Duration: Jan 28 2017 → Jan 30 2017

Other

Other	Adaptive Optics and Wavefront Control for Biological Systems III
Country	United States
City	San Francisco
Period	1/28/17 → 1/30/17

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Keywords

Adaptive Optics
Brain Imaging
Deconvolution
Extended Depth of Field
Fluorescence Microscopy
Neuron Imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Cite this

Shain, W. J., Vickers, N. A., Goldberg, B. B., Bifano, T., & Mertz, J. (2017). Extended depth-of-field microscopy with a high-speed deformable mirror. In J. Kubby, T. G. Bifano, & S. Gigan (Eds.), Adaptive Optics and Wavefront Control for Biological Systems III (Vol. 10073). [100730E] SPIE. https://doi.org/10.1117/12.2271502

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abstract = "Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.",

keywords = "Adaptive Optics, Brain Imaging, Deconvolution, Extended Depth of Field, Fluorescence Microscopy, Neuron Imaging",

author = "Shain, {William J.} and Vickers, {Nicholas A.} and Goldberg, {Bennett B.} and Thomas Bifano and Jerome Mertz",

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Shain, WJ, Vickers, NA, Goldberg, BB, Bifano, T & Mertz, J 2017, Extended depth-of-field microscopy with a high-speed deformable mirror. in J Kubby, TG Bifano & S Gigan (eds), Adaptive Optics and Wavefront Control for Biological Systems III. vol. 10073, 100730E, SPIE, Adaptive Optics and Wavefront Control for Biological Systems III, San Francisco, United States, 1/28/17. https://doi.org/10.1117/12.2271502

Extended depth-of-field microscopy with a high-speed deformable mirror. / Shain, William J.; Vickers, Nicholas A.; Goldberg, Bennett B.; Bifano, Thomas; Mertz, Jerome.

Adaptive Optics and Wavefront Control for Biological Systems III. ed. / Joel Kubby; Thomas G. Bifano; Sylvain Gigan. Vol. 10073 SPIE, 2017. 100730E.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Extended depth-of-field microscopy with a high-speed deformable mirror

AU - Shain, William J.

AU - Vickers, Nicholas A.

AU - Goldberg, Bennett B.

AU - Bifano, Thomas

AU - Mertz, Jerome

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

AB - Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

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KW - Brain Imaging

KW - Deconvolution

KW - Extended Depth of Field

KW - Fluorescence Microscopy

KW - Neuron Imaging

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VL - 10073

BT - Adaptive Optics and Wavefront Control for Biological Systems III

A2 - Kubby, Joel

A2 - Bifano, Thomas G.

A2 - Gigan, Sylvain

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Access to Document

10.1117/12.2271502