Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain*, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, Jerome Mertz

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Original languageEnglish (US)
Title of host publicationAdaptive Optics and Wavefront Control for Biological Systems III
EditorsJoel Kubby, Thomas G. Bifano, Sylvain Gigan
PublisherSPIE
Volume10073
ISBN (Electronic)9781510605879
DOIs
StatePublished - Jan 1 2017
EventAdaptive Optics and Wavefront Control for Biological Systems III - San Francisco, United States
Duration: Jan 28 2017Jan 30 2017

Other

OtherAdaptive Optics and Wavefront Control for Biological Systems III
CountryUnited States
CitySan Francisco
Period1/28/171/30/17

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Keywords

  • Adaptive Optics
  • Brain Imaging
  • Deconvolution
  • Extended Depth of Field
  • Fluorescence Microscopy
  • Neuron Imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Shain, W. J., Vickers, N. A., Goldberg, B. B., Bifano, T., & Mertz, J. (2017). Extended depth-of-field microscopy with a high-speed deformable mirror. In J. Kubby, T. G. Bifano, & S. Gigan (Eds.), Adaptive Optics and Wavefront Control for Biological Systems III (Vol. 10073). [100730E] SPIE. https://doi.org/10.1117/12.2271502