Extended host range of a β-related corynebacteriophage

Nicholas P. Cianciotto*, Neal B. Groman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Unlike most β-related phages isolated from Corynebacterium diphtheriae, phage 782 readily plaqued on strains of C. ulcerans and C. pseudotuberculosis. The extended host range of phage 782 was not, however, due to a greater ability of the phage to adsorb to bacterial surfaces. Using chemical mutagensis, a number of phage mutants were isolated which had diminished capacities to infect C. ulcerans, suggesting the existence of a locus (ehr) for extended host range. Pre-lysogenization of C. ulcerans strains with phage 782, but not its mutant form or other β-related phages, rendered them susceptible to infection by previously excluded phages. An examination of recombinants between phages 782, π, and β localized ehr to a 7 kilobase region of DNA including attP. The data are compatible with the notion that ehr encodes an anti-restriction function.

Original languageEnglish (US)
Pages (from-to)221-225
Number of pages5
JournalFEMS Microbiology Letters
Volume140
Issue number2-3
DOIs
StatePublished - Jul 1 1996

Keywords

  • Anti-restriction
  • Corynebacterium diphtheriae
  • Corynebacterium pseudotuberculosis
  • Corynebacterium ulcerans
  • Corynephage host range
  • β-related phage

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Extended host range of a β-related corynebacteriophage'. Together they form a unique fingerprint.

Cite this