Abstract
Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1617-1624 |
| Number of pages | 8 |
| Journal | Child's Nervous System |
| Volume | 32 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1 2016 |
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Keywords
- Craniopharyngiomas
- miR-132
- miR-596
- miRNA
- microRNA
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Clinical Neurology
Cite this
}
Extensive miRNA expression analysis in craniopharyngiomas. / Samis, Jill; Vanin, Elio F.; Sredni, Simone Treiger; de Bonaldo, Maria de Fátima; Costa, Fabricio F.; Tomita, Tadanori; Habiby, Reema; Zimmerman, Donald; Soares, Marcelo B.
In: Child's Nervous System, Vol. 32, No. 9, 01.09.2016, p. 1617-1624.Research output: Contribution to journal › Article
TY - JOUR
T1 - Extensive miRNA expression analysis in craniopharyngiomas
AU - Samis, Jill
AU - Vanin, Elio F.
AU - Sredni, Simone Treiger
AU - de Bonaldo, Maria de Fátima
AU - Costa, Fabricio F.
AU - Tomita, Tadanori
AU - Habiby, Reema
AU - Zimmerman, Donald
AU - Soares, Marcelo B.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.
AB - Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.
KW - Craniopharyngiomas
KW - miR-132
KW - miR-596
KW - miRNA
KW - microRNA
UR - http://www.scopus.com/inward/record.url?scp=84976273210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84976273210&partnerID=8YFLogxK
U2 - 10.1007/s00381-016-3131-1
DO - 10.1007/s00381-016-3131-1
M3 - Article
C2 - 27272948
AN - SCOPUS:84976273210
VL - 32
SP - 1617
EP - 1624
JO - Child's Nervous System
JF - Child's Nervous System
SN - 0256-7040
IS - 9
ER -