Extensive miRNA expression analysis in craniopharyngiomas

Jill Hibishman Samis, Elio F. Vanin, Simone Trieger Sredni*, Maria de Fátima de Bonaldo, Fabricio F. Costa, Tadanori Tomita, Reema L Habiby, Donald Zimmerman, Marcelo B. Soares

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.

Original languageEnglish (US)
Pages (from-to)1617-1624
Number of pages8
JournalChild's Nervous System
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Craniopharyngioma
MicroRNAs
Autopsy
Untranslated RNA
Gene Regulatory Networks
Disease Progression
Down-Regulation
Research
Neoplasms

Keywords

  • Craniopharyngiomas
  • miR-132
  • miR-596
  • miRNA
  • microRNA

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Cite this

Samis, Jill Hibishman ; Vanin, Elio F. ; Sredni, Simone Trieger ; de Bonaldo, Maria de Fátima ; Costa, Fabricio F. ; Tomita, Tadanori ; Habiby, Reema L ; Zimmerman, Donald ; Soares, Marcelo B. / Extensive miRNA expression analysis in craniopharyngiomas. In: Child's Nervous System. 2016 ; Vol. 32, No. 9. pp. 1617-1624.
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abstract = "Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan{\circledR} Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.",
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Extensive miRNA expression analysis in craniopharyngiomas. / Samis, Jill Hibishman; Vanin, Elio F.; Sredni, Simone Trieger; de Bonaldo, Maria de Fátima; Costa, Fabricio F.; Tomita, Tadanori; Habiby, Reema L; Zimmerman, Donald; Soares, Marcelo B.

In: Child's Nervous System, Vol. 32, No. 9, 01.09.2016, p. 1617-1624.

Research output: Contribution to journalArticle

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T1 - Extensive miRNA expression analysis in craniopharyngiomas

AU - Samis, Jill Hibishman

AU - Vanin, Elio F.

AU - Sredni, Simone Trieger

AU - de Bonaldo, Maria de Fátima

AU - Costa, Fabricio F.

AU - Tomita, Tadanori

AU - Habiby, Reema L

AU - Zimmerman, Donald

AU - Soares, Marcelo B.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.

AB - Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke’s pouch and are considered a “developmental disease.” microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome. Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA). Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial–mesenchymal transition. Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.

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