Extreme bendability of DNA less than 100 base pairs long revealed by single-molecule cyclization

Reza Vafabakhsh, Taekjip Ha*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

195 Scopus citations

Abstract

The classical view of DNA posits that DNA must be stiff below the persistence length [<150 base pairs (bp)], but recent studies addressing this have yielded contradictory results. We developed a fluorescence-based, protein-free assay for studying the cyclization of single DNA molecules in real time. The assay samples the equilibrium population of a sharply bent, transient species that is entirely suppressed in single-molecule mechanical measurements and is biologically more relevant than the annealed species sampled in the traditional ligase-based assay. The looping rate has a weak length dependence between 67 and 106 bp that cannot be described by the worm-like chain model. Many biologically important protein-DNA interactions that involve looping and bending of DNA below 100 bp likely use this intrinsic bendability of DNA.

Original languageEnglish (US)
Pages (from-to)1097-1101
Number of pages5
JournalScience
Volume337
Issue number6098
DOIs
StatePublished - Aug 31 2012

ASJC Scopus subject areas

  • General

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