Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft

Connor W. McCarthy, Danielle C. Ahrens, David Joda, Tyler E. Curtis, Patrick K. Bowen, Roger J. Guillory, Shu Qian Liu, Feng Zhao, Megan C. Frost, Jeremy Goldman*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although significant advances have been made in the development of artificial vascular grafts, small-diameter grafts still suffer from excessive platelet activation, thrombus formation, smooth muscle cell intimal hyperplasia, and high occurrences of restenosis. Recent discoveries demonstrating the excellent blood-contacting properties of the natural elastic lamina have raised the possibility that an acellular elastic lamina could effectively serve as a patent blood-contacting surface in engineered vascular grafts. However, the elastic lamina alone lacks the requisite mechanical properties to function as a viable vascular graft. Here, we have screened a wide range of biodegradable and biostable medical-grade polymers for their ability to adhere to the outer surface of the elastic lamina and allow cellular repopulation following engraftment in the rat abdominal aorta. We demonstrate a novel method for the fabrication of elastic lamina-polymeric hybrid small-diameter vascular grafts and identify poly(ether urethane) (PEU 1074A) as ideal for this purpose. In vivo results demonstrate graft patency over 21 days, with low thrombus formation, mild inflammation, and the general absence of smooth muscle cell hyperplasia on the graft's luminal surface. The results provide a new direction for developing small-diameter vascular grafts that are mass-producible, shelf-stable, and universally compatible due to a lack of immune response and inhibit the in-graft restenosis response that is common to nonautologous materials.

Original languageEnglish (US)
Pages (from-to)16202-16212
Number of pages11
JournalACS Applied Materials and Interfaces
Volume7
Issue number30
DOIs
StatePublished - Aug 5 2015

Fingerprint

Grafts
Fabrication
Muscle
Blood
Cells
Urethane
Platelets
Ether
Rats
Ethers
Polymers
Chemical activation
Mechanical properties

Keywords

  • blood-contacting
  • elastic lamina
  • neointimal hyperplasia
  • polymeric scaffold
  • restenosis
  • small-diameter vascular graft

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

McCarthy, C. W., Ahrens, D. C., Joda, D., Curtis, T. E., Bowen, P. K., Guillory, R. J., ... Goldman, J. (2015). Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft. ACS Applied Materials and Interfaces, 7(30), 16202-16212. https://doi.org/10.1021/acsami.5b03892
McCarthy, Connor W. ; Ahrens, Danielle C. ; Joda, David ; Curtis, Tyler E. ; Bowen, Patrick K. ; Guillory, Roger J. ; Liu, Shu Qian ; Zhao, Feng ; Frost, Megan C. ; Goldman, Jeremy. / Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft. In: ACS Applied Materials and Interfaces. 2015 ; Vol. 7, No. 30. pp. 16202-16212.
@article{9a41fc2fa3724b939ae324b982aa6dcc,
title = "Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft",
abstract = "Although significant advances have been made in the development of artificial vascular grafts, small-diameter grafts still suffer from excessive platelet activation, thrombus formation, smooth muscle cell intimal hyperplasia, and high occurrences of restenosis. Recent discoveries demonstrating the excellent blood-contacting properties of the natural elastic lamina have raised the possibility that an acellular elastic lamina could effectively serve as a patent blood-contacting surface in engineered vascular grafts. However, the elastic lamina alone lacks the requisite mechanical properties to function as a viable vascular graft. Here, we have screened a wide range of biodegradable and biostable medical-grade polymers for their ability to adhere to the outer surface of the elastic lamina and allow cellular repopulation following engraftment in the rat abdominal aorta. We demonstrate a novel method for the fabrication of elastic lamina-polymeric hybrid small-diameter vascular grafts and identify poly(ether urethane) (PEU 1074A) as ideal for this purpose. In vivo results demonstrate graft patency over 21 days, with low thrombus formation, mild inflammation, and the general absence of smooth muscle cell hyperplasia on the graft's luminal surface. The results provide a new direction for developing small-diameter vascular grafts that are mass-producible, shelf-stable, and universally compatible due to a lack of immune response and inhibit the in-graft restenosis response that is common to nonautologous materials.",
keywords = "blood-contacting, elastic lamina, neointimal hyperplasia, polymeric scaffold, restenosis, small-diameter vascular graft",
author = "McCarthy, {Connor W.} and Ahrens, {Danielle C.} and David Joda and Curtis, {Tyler E.} and Bowen, {Patrick K.} and Guillory, {Roger J.} and Liu, {Shu Qian} and Feng Zhao and Frost, {Megan C.} and Jeremy Goldman",
year = "2015",
month = "8",
day = "5",
doi = "10.1021/acsami.5b03892",
language = "English (US)",
volume = "7",
pages = "16202--16212",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "30",

}

McCarthy, CW, Ahrens, DC, Joda, D, Curtis, TE, Bowen, PK, Guillory, RJ, Liu, SQ, Zhao, F, Frost, MC & Goldman, J 2015, 'Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft', ACS Applied Materials and Interfaces, vol. 7, no. 30, pp. 16202-16212. https://doi.org/10.1021/acsami.5b03892

Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft. / McCarthy, Connor W.; Ahrens, Danielle C.; Joda, David; Curtis, Tyler E.; Bowen, Patrick K.; Guillory, Roger J.; Liu, Shu Qian; Zhao, Feng; Frost, Megan C.; Goldman, Jeremy.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 30, 05.08.2015, p. 16202-16212.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fabrication and Short-Term in Vivo Performance of a Natural Elastic Lamina-Polymeric Hybrid Vascular Graft

AU - McCarthy, Connor W.

AU - Ahrens, Danielle C.

AU - Joda, David

AU - Curtis, Tyler E.

AU - Bowen, Patrick K.

AU - Guillory, Roger J.

AU - Liu, Shu Qian

AU - Zhao, Feng

AU - Frost, Megan C.

AU - Goldman, Jeremy

PY - 2015/8/5

Y1 - 2015/8/5

N2 - Although significant advances have been made in the development of artificial vascular grafts, small-diameter grafts still suffer from excessive platelet activation, thrombus formation, smooth muscle cell intimal hyperplasia, and high occurrences of restenosis. Recent discoveries demonstrating the excellent blood-contacting properties of the natural elastic lamina have raised the possibility that an acellular elastic lamina could effectively serve as a patent blood-contacting surface in engineered vascular grafts. However, the elastic lamina alone lacks the requisite mechanical properties to function as a viable vascular graft. Here, we have screened a wide range of biodegradable and biostable medical-grade polymers for their ability to adhere to the outer surface of the elastic lamina and allow cellular repopulation following engraftment in the rat abdominal aorta. We demonstrate a novel method for the fabrication of elastic lamina-polymeric hybrid small-diameter vascular grafts and identify poly(ether urethane) (PEU 1074A) as ideal for this purpose. In vivo results demonstrate graft patency over 21 days, with low thrombus formation, mild inflammation, and the general absence of smooth muscle cell hyperplasia on the graft's luminal surface. The results provide a new direction for developing small-diameter vascular grafts that are mass-producible, shelf-stable, and universally compatible due to a lack of immune response and inhibit the in-graft restenosis response that is common to nonautologous materials.

AB - Although significant advances have been made in the development of artificial vascular grafts, small-diameter grafts still suffer from excessive platelet activation, thrombus formation, smooth muscle cell intimal hyperplasia, and high occurrences of restenosis. Recent discoveries demonstrating the excellent blood-contacting properties of the natural elastic lamina have raised the possibility that an acellular elastic lamina could effectively serve as a patent blood-contacting surface in engineered vascular grafts. However, the elastic lamina alone lacks the requisite mechanical properties to function as a viable vascular graft. Here, we have screened a wide range of biodegradable and biostable medical-grade polymers for their ability to adhere to the outer surface of the elastic lamina and allow cellular repopulation following engraftment in the rat abdominal aorta. We demonstrate a novel method for the fabrication of elastic lamina-polymeric hybrid small-diameter vascular grafts and identify poly(ether urethane) (PEU 1074A) as ideal for this purpose. In vivo results demonstrate graft patency over 21 days, with low thrombus formation, mild inflammation, and the general absence of smooth muscle cell hyperplasia on the graft's luminal surface. The results provide a new direction for developing small-diameter vascular grafts that are mass-producible, shelf-stable, and universally compatible due to a lack of immune response and inhibit the in-graft restenosis response that is common to nonautologous materials.

KW - blood-contacting

KW - elastic lamina

KW - neointimal hyperplasia

KW - polymeric scaffold

KW - restenosis

KW - small-diameter vascular graft

UR - http://www.scopus.com/inward/record.url?scp=84938613732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938613732&partnerID=8YFLogxK

U2 - 10.1021/acsami.5b03892

DO - 10.1021/acsami.5b03892

M3 - Article

C2 - 26204095

AN - SCOPUS:84938613732

VL - 7

SP - 16202

EP - 16212

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 30

ER -