Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Heeseon An; Alexander V. Statsyuk

doi:10.1039/c5cc08592f

Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Heeseon An, Alexander V. Statsyuk^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.

Original language	English (US)
Pages (from-to)	2477-2480
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	12
DOIs	https://doi.org/10.1039/c5cc08592f
State	Published - Jan 1 2016

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.",

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AU - Statsyuk, Alexander V.

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AB - We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.

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Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Abstract

ASJC Scopus subject areas

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