Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Heeseon An, Alexander V. Statsyuk*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.

Original languageEnglish (US)
Pages (from-to)2477-2480
Number of pages4
JournalChemical Communications
Volume52
Issue number12
DOIs
StatePublished - Jan 1 2016

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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