TY - JOUR
T1 - Factor XIIIa-catalyzed cross-linking of platelet and muscle actin regulation by nucleotides
AU - Cohen, Isaac
AU - Blankenberg, Tikoes A.
AU - Borden, Dennis
AU - Kahn, David R.
AU - Veis, Arthur
N1 - Funding Information:
We are grateful to Professors L. Lorand and E. Margoliash (Northwestern University) for helpful advice, J.W. Fenton, II (New York State Department of Health, Albany) for supplying the highly purified a-thrombin, and to Mr. M. Gajewski and Dr. R.H. Aster from the Milwaukee Blood Center for their help in making available the supply of human blood platelets. Supported by USPHS grants HL-20106 and HL-02212 from the National Institutes of Health.
PY - 1980
Y1 - 1980
N2 - Actin from human blood platelets or rabbit skeletal muscle can serve as substrate for factor XIIIa. The latter catalyzes the incorporation of 1.5-2 mol monodansylcadaverine/mol rabbit actin and 0.5 mol/mol platelet actin. Highly cross-linked platelet and muscle actin polymers form in the absence of added amines, indicating the presence of both acceptor and donor sites. As expected, the cross-link was found to be a γ-glutamyl-ε{lunate}-lysine bond, with an average of 0.3-0.4 mol dipeptide/mol platelet actin. Both cross-linking and amine incorporation are prevented by ATP, ADP, GTP, CTP, but not by AMP and cyclic AMP. These nucleotides may have important regulatory role in muscle and non-muscle systems.
AB - Actin from human blood platelets or rabbit skeletal muscle can serve as substrate for factor XIIIa. The latter catalyzes the incorporation of 1.5-2 mol monodansylcadaverine/mol rabbit actin and 0.5 mol/mol platelet actin. Highly cross-linked platelet and muscle actin polymers form in the absence of added amines, indicating the presence of both acceptor and donor sites. As expected, the cross-link was found to be a γ-glutamyl-ε{lunate}-lysine bond, with an average of 0.3-0.4 mol dipeptide/mol platelet actin. Both cross-linking and amine incorporation are prevented by ATP, ADP, GTP, CTP, but not by AMP and cyclic AMP. These nucleotides may have important regulatory role in muscle and non-muscle systems.
KW - (Platelet, Muscle)
KW - Actin
KW - Cross linking
KW - Factor XIIIa
KW - Nucleotide regulation
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U2 - 10.1016/0304-4165(80)90386-4
DO - 10.1016/0304-4165(80)90386-4
M3 - Article
C2 - 6892787
AN - SCOPUS:0018819777
SN - 0304-4165
VL - 628
SP - 365
EP - 375
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - C
ER -