Failure of 5-HT3 receptors in regulation of ethanol-induced ascorbic acid release in rat striatum

Chun Fu Wu*, Jing Liu, Wen Liu, Silvana Consolo, Mei Huang, Jing Yu Yang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Previous studies have shown that the serotonergic system was involved in ethanol-induced striatal ascorbic acid release in rats. In the present study the possible role of 5-HT3 receptors in ethanol-induced striatal ascorbic acid release was investigated in rats using 5-HT3 antagonists ondansetron, DAU 6215 and 5-HT3 agonist 2-methyl-serotonin. Extracellular level of ascorbic acid in the striatum was determined by means of in vivo microdialysis coupled to HPLC with electrochemical detection. Ethanol (3 g/kg, i.p.) induced a significant increase in ascorbic acid release. Ondansetron (0.2 and 2.0 mg/kg, i.p.), DAU 6215 (0.06, 0.12 and 0.24 mg/kg, i.p.) and 2-methyl-serotonin (250 μg/rat, i.c.v.), administered 10 minutes before 0.15 M NaCl or ethanol (3 g/kg, i.p.), affect neither the basal nor the ethanol-induced ascorbic acid release in rat striatum. 2-Methyl-serotonin, at a dose of 500 μg/rat, i.c.v., increased the basal, but did not affect the ethanol-induced ascorbic acid release in rat striatum. However, ritanserin (1 mg/kg, s.c.), a 5-HT2 receptor antagonist, and BIMU 8 (40 μg/rat, i.c.v.), a 5-HT4 agonist, significantly antagonized ethanol-induced ascorbic acid release. These results suggest that 5-HT3 receptors, which form a part of cation channels, may not be involved in ethanol-induced striatal ascorbic acid release.

Original languageEnglish (US)
Pages (from-to)25-34
Number of pages10
JournalAddiction Biology
Volume6
Issue number1
DOIs
StatePublished - Feb 5 2001

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology
  • Psychiatry and Mental health

Fingerprint Dive into the research topics of 'Failure of 5-HT<sub>3</sub> receptors in regulation of ethanol-induced ascorbic acid release in rat striatum'. Together they form a unique fingerprint.

Cite this