Failure of simian virus 40 small t antigen to disorganize actin cables in nonpermissive cell lines

B. Phillips, K. Rundell

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Mouse C3H 10T1/2 cell lines expressing the simian virus 40 (SV40) small t antigen were obtained by cotransfection of pSV2neo and plasmids which encode small t. Cell lines derived from two plasmids which encode small t in the absence of stable deletion fragments of the large T antigen were morphologically normal and grew to slightly higher saturation densities in low serum than control cell lines. Unexpectedly, the clones had highly organized actin cables, as did parental 10T1/2 cells infected with wild-type SV40. These observations and comparisons of rat F111 cells infected with either polyomavirus or SV40 suggest that the SV40 small t antigen does not directly affect cytoskeletal organization.

Original languageEnglish (US)
Pages (from-to)768-775
Number of pages8
JournalJournal of virology
Volume62
Issue number3
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Failure of simian virus 40 small t antigen to disorganize actin cables in nonpermissive cell lines'. Together they form a unique fingerprint.

Cite this