Familial C3 glomerulonephritis caused by a novel CFHR5-CFHR2 fusion gene

Xue Xiao, Cybele Ghossein, Agustín Tortajadam, Yuzhou Zhang, Nicole Meyer, Michael Jones, Nicolo Ghiringhelli Borsa, Carla M. Nester, Christie P. Thomas, Santiago Rodríquez de Córdoba, Richard J.H. Smith*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

C3 glomerulopathy (C3G) is an ultra-rare complement-mediated renal disease characterized histologically by the predominance of C3 deposition within in the glomerulus. Familial cases of C3G are extremely uncommon and offer unique insight into the genetic drivers of complement dysregulation. In this report, we describe a patient who presented with C3G. Because a relative carried the same diagnosis, we sought an underlying genetic commonality to explain the phenotype. As part of a comprehension genetic screen, we completed multiplex ligation-dependent probe amplification across the complement factor H related region and identified amplification alterations consistent with a genomic rearrangement. Using comparative genomic hybridization, we narrowed and then cloned the rearrangement breakpoints thereby defining a novel fusion gene that is translated into a serum protein comprised of factor H related-5 (short consensus repeats 1 and 2) and factor H-related-2 (short consensus repeats 1–4). These data highlight the role of factor H related proteins in the control of complement activity and illustrate how perturbation of that control leads to C3G.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalMolecular Immunology
Volume77
DOIs
StatePublished - Sep 1 2016

Keywords

  • C3 glomerulopathy
  • Complement factor H related-2
  • Complement factor H related-5

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Familial C3 glomerulonephritis caused by a novel CFHR5-CFHR2 fusion gene'. Together they form a unique fingerprint.

  • Cite this

    Xiao, X., Ghossein, C., Tortajadam, A., Zhang, Y., Meyer, N., Jones, M., Borsa, N. G., Nester, C. M., Thomas, C. P., de Córdoba, S. R., & Smith, R. J. H. (2016). Familial C3 glomerulonephritis caused by a novel CFHR5-CFHR2 fusion gene. Molecular Immunology, 77, 89-96. https://doi.org/10.1016/j.molimm.2016.07.007