Far-Red Photoactivatable BODIPYs for the Super-Resolution Imaging of Live Cells

Yang Zhang, Ki Hee Song, Sicheng Tang, Laura Ravelo, Janet Cusido, Cheng Sun, Hao F. Zhang*, Françisco M. Raymo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


The photoinduced disconnection of an oxazine heterocycle from a borondipyrromethene (BODIPY) chromophore activates bright far-red fluorescence. The high brightness of the product and the lack of autofluorescence in this spectral region allow its detection at the single-molecule level within the organelles of live cells. Indeed, these photoactivatable fluorophores localize in lysosomal compartments and remain covalently immobilized within these organelles. The suppression of diffusion allows the reiterative reconstruction of subdiffraction images and the visualization of the labeled organelles with excellent localization precision. Thus, the combination of photochemical, photophysical and structural properties designed into our fluorophores enable the visualization of live cells with a spatial resolution that is inaccessible to conventional fluorescence imaging.

Original languageEnglish (US)
Pages (from-to)12741-12745
Number of pages5
JournalJournal of the American Chemical Society
Issue number40
StatePublished - Oct 10 2018

ASJC Scopus subject areas

  • General Chemistry
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry


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