Fast low-angle positive contrast steady-state free precession imaging of USPIO-labeled macrophages: theory and in vitro experiment

Nicole Mascheri*, Rohan Dharmakumar, Zhuoli Zhang, Tatjana Paunesku, Gayle Woloschak, Debiao Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The feasibility of imaging macrophages labeled with ultrasmall superparamagnetic iron-oxide nanoparticles (USPIO) with fast low-angle positive contrast steady-state free precession (FLAPS) was investigated through theory and in vitro experiment. Human macrophage cells were labeled with USPIO and imaged at 1.5 T. The metric "visibility," which combines magnitude and spatial extent of positive contrast, was used to evaluate the images. Negative contrast steady-state free precession (SSFP) and gradient-echo (GRE) imaging were also evaluated. Positive contrast was observed for relatively high concentrations of labeled cells for flip angles less than α=25°. Theoretical and experimental results indicate that positive visibility (VISPOS) was maximized at α=10° and 15°. Low flip angle SSFP also provided negative contrast comparable to standard SSFP and GRE imaging. Results suggest that USPIO-labeled macrophages are capable of producing the conditions necessary for positive contrast with FLAPS at clinical field strength (1.5 T) and resolution (0.8×0.8×3 mm3).

Original languageEnglish (US)
Pages (from-to)961-969
Number of pages9
JournalMagnetic Resonance Imaging
Volume27
Issue number7
DOIs
StatePublished - Sep 2009

Keywords

  • Macrophages
  • Positive contrast
  • SSFP
  • USPIO

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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