Abstract
Mononuclear phagocytes, including monocytes, macrophages, and dendritic cells, contribute to tissue integrity as well as to innate and adaptive immune defense. Emerging evidence for labor division indicates that manipulation of these cells could bear therapeutic potential. However, specific ontogenies of individual populations and the overall functional organization of this cellular network are not well defined. Here we report a fate-mapping study of the murine monocyte and macrophage compartment taking advantage of constitutive and conditional CX3CR1 promoter-driven Cre recombinase expression. We have demonstrated that major tissue-resident macrophage populations, including liver Kupffer cells and lung alveolar, splenic, and peritoneal macrophages, are established prior to birth and maintain themselves subsequently during adulthood independent of replenishment by blood monocytes. Furthermore, we have established that short-lived Ly6C+ monocytes constitute obligatory steady-state precursors of blood-resident Ly6C- cells and that the abundance of Ly6C+ blood monocytes dynamically controls the circulation lifespan of their progeny.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-91 |
| Number of pages | 13 |
| Journal | Immunity |
| Volume | 38 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 24 2013 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases
Cite this
}
Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis. / Yona, Simon; Kim, Ki Wook; Wolf, Yochai; Mildner, Alexander; Varol, Diana; Breker, Michal; Strauss-Ayali, Dalit; Viukov, Sergey; Guilliams, Martin; Misharin, Alexander; Hume, David A.; Perlman, Harris R; Malissen, Bernard; Zelzer, Elazar; Jung, Steffen.
In: Immunity, Vol. 38, No. 1, 24.01.2013, p. 79-91.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis
AU - Yona, Simon
AU - Kim, Ki Wook
AU - Wolf, Yochai
AU - Mildner, Alexander
AU - Varol, Diana
AU - Breker, Michal
AU - Strauss-Ayali, Dalit
AU - Viukov, Sergey
AU - Guilliams, Martin
AU - Misharin, Alexander
AU - Hume, David A.
AU - Perlman, Harris R
AU - Malissen, Bernard
AU - Zelzer, Elazar
AU - Jung, Steffen
PY - 2013/1/24
Y1 - 2013/1/24
N2 - Mononuclear phagocytes, including monocytes, macrophages, and dendritic cells, contribute to tissue integrity as well as to innate and adaptive immune defense. Emerging evidence for labor division indicates that manipulation of these cells could bear therapeutic potential. However, specific ontogenies of individual populations and the overall functional organization of this cellular network are not well defined. Here we report a fate-mapping study of the murine monocyte and macrophage compartment taking advantage of constitutive and conditional CX3CR1 promoter-driven Cre recombinase expression. We have demonstrated that major tissue-resident macrophage populations, including liver Kupffer cells and lung alveolar, splenic, and peritoneal macrophages, are established prior to birth and maintain themselves subsequently during adulthood independent of replenishment by blood monocytes. Furthermore, we have established that short-lived Ly6C+ monocytes constitute obligatory steady-state precursors of blood-resident Ly6C- cells and that the abundance of Ly6C+ blood monocytes dynamically controls the circulation lifespan of their progeny.
AB - Mononuclear phagocytes, including monocytes, macrophages, and dendritic cells, contribute to tissue integrity as well as to innate and adaptive immune defense. Emerging evidence for labor division indicates that manipulation of these cells could bear therapeutic potential. However, specific ontogenies of individual populations and the overall functional organization of this cellular network are not well defined. Here we report a fate-mapping study of the murine monocyte and macrophage compartment taking advantage of constitutive and conditional CX3CR1 promoter-driven Cre recombinase expression. We have demonstrated that major tissue-resident macrophage populations, including liver Kupffer cells and lung alveolar, splenic, and peritoneal macrophages, are established prior to birth and maintain themselves subsequently during adulthood independent of replenishment by blood monocytes. Furthermore, we have established that short-lived Ly6C+ monocytes constitute obligatory steady-state precursors of blood-resident Ly6C- cells and that the abundance of Ly6C+ blood monocytes dynamically controls the circulation lifespan of their progeny.
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UR - http://www.scopus.com/inward/citedby.url?scp=84872765982&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2012.12.001
DO - 10.1016/j.immuni.2012.12.001
M3 - Article
VL - 38
SP - 79
EP - 91
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 1
ER -