Fate of the initial follicle pool: Empirical and mathematical evidence supporting its sufficiency for adult fertility

Sarah K. Bristol-Gould, Pamela K. Kreeger, Christina G. Selkirk, Signe M. Kilen, Kelly E. Mayo, Lonnie D. Shea, Teresa K. Woodruff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

The importance of the initial follicle pool in fertility in female adult mammals has recently been debated. Utilizing a mathematical model of the dynamics of follicle progression (primordial to primary to secondary), we examined whether the initial follicle pool is sufficient for adult fertility through reproductive senescence in CD1 mice. Follicles in each stage were counted from postnatal day 6 through 12 months and data were fit to a series of first-order differential equations representing two mechanisms: an initial pool of primordial follicles as the only follicle source (fixed pool model), or an initial primordial follicle pool supplemented by germline stem cells (stem cell model). The fixed pool model fit the experimental data, accurately representing the maximum observed primary follicle number reached by 4-6 months of age. Although no germline stem cells could be identified by SSEA-1 immunostaining, the stem cell model was tested using a range of de novo primordial follicle production rates. The stem cell model failed to describe the observed decreases in follicles over time and did not parallel the accumulation and subsequent reduction in primary follicles during the early fertile lifespan of the mouse. Our results agree with established dogma that the initial endowment of ovarian follicles is not supplemented by an appreciable number of stem cells; rather, it is sufficient to ensure the fertility needs of the adult mouse.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalDevelopmental Biology
Volume298
Issue number1
DOIs
StatePublished - Oct 1 2006

Keywords

  • Follicle counts
  • Germ cells
  • Germline stem cells
  • Oocyte
  • Primordial follicles
  • SSEA-1

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Fate of the initial follicle pool: Empirical and mathematical evidence supporting its sufficiency for adult fertility'. Together they form a unique fingerprint.

Cite this