TY - JOUR
T1 - Fatty acid ethyl ester synthesis by the isolated perfused rat heart
AU - Chang, Wanlin
AU - Waltenbaugh, Carl
AU - Borensztajn, Jayme
N1 - Funding Information:
From the Departments of Pathology and Microbiology-Immunology, Northwestern University Medical School, Chicago, IL. Submitted October 7, 1996; accepted February 21, 1997. Supported by grants from the National Institutes of Health, National Institute on Alcohol Abuse and Alcoholism (AA-08275), the Sidney and Bess Eisenberg Memorial Fund, and the Feinberg Cardiovascular Research Institute. Address reprint requests to Jayme Borensztajn, MD, Department of Pathology, Northwestern University Medical School, 303 E Chicago Ave, Chicago, IL 60611. Copyright © 1997 by W.B. Saunders Company 0026-0495/97/4608-0013503.00/0
PY - 1997
Y1 - 1997
N2 - Fatty acid ethyl esters (FAEEs), nonoxidative by-products of ethanol metabolism, are found in various tissues and plasma after ethanol ingestion and may be responsible for some of the pathological changes observed in alcohol-consuming individuals. Previous studies demonstrated that several different enzymes, including lipoprotein lipase (LPL), can catalyze FAEE synthesis in vitro. We report that LPL cetalyzes FAEE synthesis in isolated rat hearts perfused with chylomicrons in the presence of ethanol. Most of the FAEEs accumulated in the perfusate, suggesting that in vivo, plasma FAEEs derive from LPL-mediated synthesis. Our results are the first demonstration of the direct involvement of a specific enzyme, LPL, in FAEE synthesis under physiological conditions.
AB - Fatty acid ethyl esters (FAEEs), nonoxidative by-products of ethanol metabolism, are found in various tissues and plasma after ethanol ingestion and may be responsible for some of the pathological changes observed in alcohol-consuming individuals. Previous studies demonstrated that several different enzymes, including lipoprotein lipase (LPL), can catalyze FAEE synthesis in vitro. We report that LPL cetalyzes FAEE synthesis in isolated rat hearts perfused with chylomicrons in the presence of ethanol. Most of the FAEEs accumulated in the perfusate, suggesting that in vivo, plasma FAEEs derive from LPL-mediated synthesis. Our results are the first demonstration of the direct involvement of a specific enzyme, LPL, in FAEE synthesis under physiological conditions.
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U2 - 10.1016/S0026-0495(97)90081-0
DO - 10.1016/S0026-0495(97)90081-0
M3 - Article
C2 - 9258276
AN - SCOPUS:0030877419
VL - 46
SP - 926
EP - 929
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
SN - 0026-0495
IS - 8
ER -
