TY - JOUR
T1 - Feasibility and utility of using chromosomal aneusomy to further define the cytologic categories in nipple aspirate fluid specimens
T2 - A preliminary study
AU - Krishnamurthy, Savitri
AU - Zhao, Lian
AU - Hayes, Kimberly
AU - Glassman, Armand B.
AU - Cristofanilli, Massimo
AU - Singletary, S. Eva
AU - Hunt, Kelly K.
AU - Kuerer, Henry M.
AU - Sneige, Nour
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/10/25
Y1 - 2004/10/25
N2 - BACKGROUND. There is renewed interest in using the cytologic changes in the epithelial cells obtained from specimens such as nipple aspiration fluid (NAF) and ductal lavage for risk stratification of women at increased risk for developing breast carcinoma. METHODS. Molecular tests such as fluorescence in situ hybridization (FISH) have the potential to be used as adjuncts to conventional cytology for more accurately categorizing cells in these types of specimens. The current study investigated the feasibility and utility of FISH analysis of aneusomy in chromosomes 1, 8, 11 and 17 as an adjunct to conventional cytology in the classification of NAF specimens. RESULTS. The authors found chromosomal aneusomy for at least one chromosome in all three malignant and both markedly atypical cases. Of the five cases classified as being mildy atypical on cytology, four were disomic, and only one showed aneusomy in chromosomes 8 and 11. CONCLUSIONS. The current study established the possibilities, limitations, and feasibility of using FISH in conjunction with routine cytology for a more accurate classification of ductal epithelial cells in NAF specimens. FISH-based detection of chromosomal aneusomy helped to define mild atypia, thereby aiding in the selection of the truly atypical cases for appropriate therapeutic intervention. In addition, FISH-based detection of chromosomal aneusomy can also be a valuable adjunct to conventional cytology in selected cases for confirming a benign, suspicious, or malignant diagnosis.
AB - BACKGROUND. There is renewed interest in using the cytologic changes in the epithelial cells obtained from specimens such as nipple aspiration fluid (NAF) and ductal lavage for risk stratification of women at increased risk for developing breast carcinoma. METHODS. Molecular tests such as fluorescence in situ hybridization (FISH) have the potential to be used as adjuncts to conventional cytology for more accurately categorizing cells in these types of specimens. The current study investigated the feasibility and utility of FISH analysis of aneusomy in chromosomes 1, 8, 11 and 17 as an adjunct to conventional cytology in the classification of NAF specimens. RESULTS. The authors found chromosomal aneusomy for at least one chromosome in all three malignant and both markedly atypical cases. Of the five cases classified as being mildy atypical on cytology, four were disomic, and only one showed aneusomy in chromosomes 8 and 11. CONCLUSIONS. The current study established the possibilities, limitations, and feasibility of using FISH in conjunction with routine cytology for a more accurate classification of ductal epithelial cells in NAF specimens. FISH-based detection of chromosomal aneusomy helped to define mild atypia, thereby aiding in the selection of the truly atypical cases for appropriate therapeutic intervention. In addition, FISH-based detection of chromosomal aneusomy can also be a valuable adjunct to conventional cytology in selected cases for confirming a benign, suspicious, or malignant diagnosis.
KW - Breast carcinoma
KW - Chromosomal aneusomy
KW - Fluorescence in situ hybridization
KW - Nipple aspirate fluid
UR - http://www.scopus.com/inward/record.url?scp=5644273990&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=5644273990&partnerID=8YFLogxK
U2 - 10.1002/cncr.20558
DO - 10.1002/cncr.20558
M3 - Article
C2 - 15376199
AN - SCOPUS:5644273990
SN - 0008-543X
VL - 102
SP - 322
EP - 327
JO - cancer
JF - cancer
IS - 5
ER -