Feminized tassels of maize mop1 and ts1 mutants exhibit altered levels of miR156 and specific SBP-box genes

Judd F. Hultquist, Jane E. Dorweiler

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Development of the unisexual maize inflorescences requires the abortion of pistillate primordia in the florets of the developing tassel and the arrest of staminate primordia in the florets of the developing ears. Mutations of many genes that lie within this sexual differentiation pathway, such as tasselseed1 (ts1), or that influence this pathway, such as mediator of paramutation 1 (mop1), result in feminization of the normally male tassel. Here, we show the loss of mop1 or ts1 function results in increased mRNA levels for several members of the SBP-box gene family. Our analyses of this family expand the number of maize SBP-box genes from 9 to 31 members. Intron-exon structures as well as phylogenetic data support the division of these family members into six groups. The SBP-box genes upregulated in feminized tassels fall into two groups, share common structural motifs and include the presence of a target site for miR156. Small RNA blots show miR156 levels are decreased in both mop1 and ts1 mutants. While there is a correlation between miR156 levels and SBP-box gene transcript levels, this correlation is not absolute, and thus we hypothesize that decreased levels of miR156 may provide competency for SBP-box gene upregulation by other common factors yet to be identified. We present a model that provides a putative link between ts1, ts2, ts4, Ts6, and mop1 in the sex-determination pathway.

Original languageEnglish (US)
Pages (from-to)99-113
Number of pages15
JournalPlanta
Volume229
Issue number1
DOIs
StatePublished - Dec 1 2008

Keywords

  • Inflorescence development
  • RT-PCR
  • Sex determination
  • Sexual differentiation
  • Squamosa promoter binding protein
  • Tasselseed

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Fingerprint Dive into the research topics of 'Feminized tassels of maize mop1 and ts1 mutants exhibit altered levels of miR156 and specific SBP-box genes'. Together they form a unique fingerprint.

Cite this