Fetal cells in maternal blood: Recovery by charge flow separation

Stephen S. Wachtel; David Sammons; Michael Manley; Gwendolyn Wachtel; Garland Twitty; Joseph Utermohlen; Owen P. Phillips; Lee P. Shulman; Douglas J. Taron; Uwe R. Müller; Peter Koeppen; Teresa M. Ruffalo; Karen Addis; Richard Porreco; Joyce Murata-Collins; Natalie B. Parker; Loris McGavran

doi:10.1007/s004390050181

Fetal cells in maternal blood: Recovery by charge flow separation

Stephen S. Wachtel^*, David Sammons, Michael Manley, Gwendolyn Wachtel, Garland Twitty, Joseph Utermohlen, Owen P. Phillips, Lee P. Shulman, Douglas J. Taron, Uwe R. Müller, Peter Koeppen, Teresa M. Ruffalo, Karen Addis, Richard Porreco, Joyce Murata-Collins, Natalie B. Parker, Loris McGavran

^*Corresponding author for this work

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.

Original language	English (US)
Pages (from-to)	162-166
Number of pages	5
Journal	Human Genetics
Volume	98
Issue number	2
DOIs	https://doi.org/10.1007/s004390050181
State	Published - 1996

ASJC Scopus subject areas

Genetics
Genetics(clinical)

Access to Document

10.1007/s004390050181

Cite this

Wachtel, S. S., Sammons, D., Manley, M., Wachtel, G., Twitty, G., Utermohlen, J., Phillips, O. P., Shulman, L. P., Taron, D. J., Müller, U. R., Koeppen, P., Ruffalo, T. M., Addis, K., Porreco, R., Murata-Collins, J., Parker, N. B., & McGavran, L. (1996). Fetal cells in maternal blood: Recovery by charge flow separation. Human Genetics, 98(2), 162-166. https://doi.org/10.1007/s004390050181

@article{1a54534412ce4aa1a403d46c7ea29a1d,

title = "Fetal cells in maternal blood: Recovery by charge flow separation",

abstract = "Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.",

author = "Wachtel, {Stephen S.} and David Sammons and Michael Manley and Gwendolyn Wachtel and Garland Twitty and Joseph Utermohlen and Phillips, {Owen P.} and Shulman, {Lee P.} and Taron, {Douglas J.} and M{\"u}ller, {Uwe R.} and Peter Koeppen and Ruffalo, {Teresa M.} and Karen Addis and Richard Porreco and Joyce Murata-Collins and Parker, {Natalie B.} and Loris McGavran",

year = "1996",

doi = "10.1007/s004390050181",

language = "English (US)",

volume = "98",

pages = "162--166",

journal = "Human Genetics",

issn = "0340-6717",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

}

Wachtel, SS, Sammons, D, Manley, M, Wachtel, G, Twitty, G, Utermohlen, J, Phillips, OP, Shulman, LP, Taron, DJ, Müller, UR, Koeppen, P, Ruffalo, TM, Addis, K, Porreco, R, Murata-Collins, J, Parker, NB & McGavran, L 1996, 'Fetal cells in maternal blood: Recovery by charge flow separation', Human Genetics, vol. 98, no. 2, pp. 162-166. https://doi.org/10.1007/s004390050181

TY - JOUR

T1 - Fetal cells in maternal blood

T2 - Recovery by charge flow separation

AU - Wachtel, Stephen S.

AU - Sammons, David

AU - Manley, Michael

AU - Wachtel, Gwendolyn

AU - Twitty, Garland

AU - Utermohlen, Joseph

AU - Phillips, Owen P.

AU - Shulman, Lee P.

AU - Taron, Douglas J.

AU - Müller, Uwe R.

AU - Koeppen, Peter

AU - Ruffalo, Teresa M.

AU - Addis, Karen

AU - Porreco, Richard

AU - Murata-Collins, Joyce

AU - Parker, Natalie B.

AU - McGavran, Loris

PY - 1996

Y1 - 1996

N2 - Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.

AB - Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.

UR - http://www.scopus.com/inward/record.url?scp=8944252344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8944252344&partnerID=8YFLogxK

U2 - 10.1007/s004390050181

DO - 10.1007/s004390050181

M3 - Article

C2 - 8698333

AN - SCOPUS:8944252344

SN - 0340-6717

VL - 98

SP - 162

EP - 166

JO - Human Genetics

JF - Human Genetics

IS - 2

ER -

Fetal cells in maternal blood: Recovery by charge flow separation

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this