Fiber photometry in striatum reflects primarily nonsomatic changes in calcium

Alex A. Legaria, Bridget A. Matikainen-Ankney, Ben Yang, Biafra Ahanonu, Julia A. Licholai, Jones G. Parker*, Alexxai V. Kravitz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, ‘spiking’) or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium.

Original languageEnglish (US)
Pages (from-to)1124-1128
Number of pages5
JournalNature neuroscience
Issue number9
StatePublished - Sep 2022

ASJC Scopus subject areas

  • General Neuroscience


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