Fibrillar systems in cell motility

Robert D. Goldman; Germaine Berg; Anne Bushnell; Cheng Ming Chang; Lois Dickerman; Nancy Hopkins; Mary Louise Miller; Robert Pollack; Eugenia Wang

doi:10.1002/9780470719978.ch5

Fibrillar systems in cell motility

Robert D. Goldman^*, Germaine Berg, Anne Bushnell, Cheng Ming Chang, Lois Dickerman, Nancy Hopkins, Mary Louise Miller, Robert Pollack, Eugenia Wang

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Several types of cytoplasmic fibres are found in various cultured fibroblastic and epithelial-like cells (including the BHK-21 and BSC-1 cell lines): microtubules (about 25 nm in diameter), filaments (about 10 nm in diameter) and microfilaments (about 6 nm in diameter). The distribution, coordinated activities and the dynamic nature of these fibres suggest that they function in several aspects of cell motility, including cell spreading, locomotion and changes in cell shape during the normal life cycle. Experiments with cycloheximide indicate that pools of precursors, which might be used to assemble fibres, are available in cells during cell spreading. Colchicine treatment demonstrates that microtubules are important in the formation and maintenance of the shape of fibroblastic cells, but not that of epithelial cells. The use of cytochalasin B and its interactions with the cell surface are also discussed.

Original language	English (US)
Title of host publication	Locomotion of Tissue Cells
Publisher	Wiley-Blackwell
Pages	83-107
Number of pages	25
ISBN (Electronic)	9780470719978
ISBN (Print)	9789021940151
DOIs	https://doi.org/10.1002/9780470719978.ch5
State	Published - May 30 2008

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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title = "Fibrillar systems in cell motility",

abstract = "Several types of cytoplasmic fibres are found in various cultured fibroblastic and epithelial-like cells (including the BHK-21 and BSC-1 cell lines): microtubules (about 25 nm in diameter), filaments (about 10 nm in diameter) and microfilaments (about 6 nm in diameter). The distribution, coordinated activities and the dynamic nature of these fibres suggest that they function in several aspects of cell motility, including cell spreading, locomotion and changes in cell shape during the normal life cycle. Experiments with cycloheximide indicate that pools of precursors, which might be used to assemble fibres, are available in cells during cell spreading. Colchicine treatment demonstrates that microtubules are important in the formation and maintenance of the shape of fibroblastic cells, but not that of epithelial cells. The use of cytochalasin B and its interactions with the cell surface are also discussed.",

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AU - Goldman, Robert D.

AU - Berg, Germaine

AU - Bushnell, Anne

AU - Chang, Cheng Ming

AU - Dickerman, Lois

AU - Hopkins, Nancy

AU - Miller, Mary Louise

AU - Pollack, Robert

AU - Wang, Eugenia

PY - 2008/5/30

Y1 - 2008/5/30

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