Fibrinogen in rat gastrointestinal lymph before, during and after intraduodenal administration of emulsified triglyceride: Fibrinogen bound to chylomicrons in gastrointestinal lymph is functional

Ashley P. DeAnglis, Charity M. Einhaus, Amy D. Sombun, Looi C. Ee, Gregory S. Retzinger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Samples of gastrointestinal lymph were collected from fasted, male, Sprague-Dawley rats before, during and after intraduodenal administration of either a phospholipid-stabilized, triglyceride-rich emulsion or the dextrose-saline diluent of the emulsion. In lipid-treated rats, the triglyceride, total protein, and functional fibrinogen contents of lymph increased significantly during the 4 h of continuous lipid infusion, with all analytes returning to near baseline values by 20 h later. Levels of the same analytes changed little, if at all, in control animals. As assessed using immunoblotting, chylomicrons in gastrointestinal lymph are coated with fibrinogen. Fibrinogen-coated chylomicrons readily incorporate into solution phase clots and, in the presence of thrombin, adhere in heparin-preventable fashion to each other and to other fibrinogen-coated surfaces. Taken together, these data indicate that lipid feeding creates in gastrointestinal lymph a condition that is conducive temporally to the physical association of fibrinogen with newly ingested lipids before they reach the circulatory system.

Original languageEnglish (US)
Pages (from-to)419-432
Number of pages14
JournalThrombosis research
Volume105
Issue number5
DOIs
StatePublished - Mar 1 2002

Keywords

  • Antithrombin
  • Atherosclerosis
  • Ceruloplasmin
  • Chylomicrons
  • Fibrinogen
  • Gastrointestinal lymph
  • Haptoglobin
  • Heparin
  • Postprandial lipids
  • Triglycerides

ASJC Scopus subject areas

  • Hematology

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