Fibroblast growth factor-10 upregulates Na,K-ATPase via the MAPK pathway

D. Upadhyay; E. Lecuona; A. Comellas; D. W. Kamp; J. I. Sznajder

doi:10.1016/S0014-5793(03)00527-1

Fibroblast growth factor-10 upregulates Na,K-ATPase via the MAPK pathway

D. Upadhyay^*, E. Lecuona, A. Comellas, D. W. Kamp, J. I. Sznajder

^*Corresponding author for this work

Medicine, Pulmonary Division

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and α1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.

Original language	English (US)
Pages (from-to)	173-176
Number of pages	4
Journal	FEBS Letters
Volume	545
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00527-1
State	Published - Jun 19 2003

Keywords

Alveolar epithelial cell
Fibroblast growth factor-10
Mitogen-activated protein kinase
Na,K-ATPase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Fibroblast growth factor-10 upregulates Na,K-ATPase via the MAPK pathway",

abstract = "We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and α1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.",

keywords = "Alveolar epithelial cell, Fibroblast growth factor-10, Mitogen-activated protein kinase, Na,K-ATPase",

author = "D. Upadhyay and E. Lecuona and A. Comellas and Kamp, {D. W.} and Sznajder, {J. I.}",

note = "Funding Information: Supported in part by the NRSA (D.U.), and HL48129 (J.I.S.), Veterans Administration Merit Award (D.W.K.).",

year = "2003",

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AU - Upadhyay, D.

AU - Lecuona, E.

AU - Comellas, A.

AU - Kamp, D. W.

AU - Sznajder, J. I.

N1 - Funding Information: Supported in part by the NRSA (D.U.), and HL48129 (J.I.S.), Veterans Administration Merit Award (D.W.K.).

PY - 2003/6/19

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N2 - We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and α1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.

AB - We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and α1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.

KW - Alveolar epithelial cell

KW - Fibroblast growth factor-10

KW - Mitogen-activated protein kinase

KW - Na,K-ATPase

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