Fibroblast growth factor-10 upregulates Na,K-ATPase via the MAPK pathway

D. Upadhyay*, E. Lecuona, A. Comellas, D. W. Kamp, J. I. Sznajder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and α1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U0126, a Grb2-SOS inhibitor (SH3-b-p peptide), or a Ras inhibitor (farnesyl transferase inhibitor (FTI 277)), as well as N17-AEC that express a Ras dominant negative protein each prevented FGF-10-mediated Na,K-ATPase recruitment to the AEC plasma membrane. Accordingly, we provide first evidence that FGF-10 upregulates (short-term) the Na,K-ATPase activity in AEC via the Grb2-SOS/Ras/MAPK pathway.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalFEBS Letters
Volume545
Issue number2-3
DOIs
StatePublished - Jun 19 2003

Keywords

  • Alveolar epithelial cell
  • Fibroblast growth factor-10
  • Mitogen-activated protein kinase
  • Na,K-ATPase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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