Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells

Patricia E. Burger*, Sandra Coetzee, Wallace L. McKeehan, Mikio Kan, Perry Cook, Yong Fan, Toshio Suda, Robert P. Hebbel, Nicolas Novitzky, William A. Muller, E. Lynette Wilson

*Corresponding author for this work

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Recent experiments show that hematopoietic progenitor cell populations contain endothelial precursor cells. We have isolated a population of CD34 + cells that expresses fibroblast growth factor receptor-1 (FGFR-1) and that differentiates into endothelial cells in vitro. We find that 4.5% ± 2.1% of CD34 + cells isolated from bone marrow, cord blood, and mobilized peripheral blood express FGFR-1 and that viable CD34 +FGFR + cells are small, with little granularity, and express both primitive hematopoietic and endothelial markers on their surface. The primitive hematopoietic markers AC133, c-kit, and Thy-1 are coexpressed by 75%, 85%, and 64% of CD34 +FGFR + cells, respectively. Most of the CD34 +FGFR + cells also express antigens found on endothelial cells, such as CD31, vascular endothelial growth factor receptor-2, and the endothelial-specific cell surface marker, vascular endothelial cadherin (VE-cadherin), whereas 56% to 60% of the cells express Tie, Tek, and the endothelial-specific marker, P1H12. The CD34 +FGFR + population is enriched in cells expressing endothelial-specific antigens compared with the CD34 + population. Isolated CD34 +FGFR + cells grow slowly in culture, are stimulated by fibroblast growth factor-2 and vascular endothelial growth factor, and give rise to cells that express von Willebrand factor and VE-cadherin and that incorporate acetylated low-density lipoprotein. These experiments show that FGFR-1 is expressed by a subpopulation of CD34 + cells that give rise to endothelial cells in vitro, indicating that this population contains endothelial stem/progenitor cells.

Original languageEnglish (US)
Pages (from-to)3527-3535
Number of pages9
JournalBlood
Volume100
Issue number10
DOIs
StatePublished - Nov 15 2002

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Burger, P. E., Coetzee, S., McKeehan, W. L., Kan, M., Cook, P., Fan, Y., Suda, T., Hebbel, R. P., Novitzky, N., Muller, W. A., & Lynette Wilson, E. (2002). Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells. Blood, 100(10), 3527-3535. https://doi.org/10.1182/blood.V100.10.3527