Fibronectin (FN) in hypertrophic scars and keloids

C. Ward Kischer*, Mary J.C. Hendrix

*Corresponding author for this work

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Fibronectin (FN) distribution was compared among samples of normal human dermis, hypertrophic scar, keloid, and granulation tissues from deep injuries. Localization was established by use of fibronectin antibodies and the indirect immunofluorescence method. Fresh-frozen tissue was sectioned on a cryostat and examined by epifluorescence. Hypertrophic scar and keloid demonstrated heavy deposition of FN, which conformed to the nodular characteristics of the lesions. Intense localization occurred in granulation tissue over fibroblasts which were stellate and vesiculated, and over small blood vessels. FN-staining was weak in areas over fibroblasts which were more rounded and nonvesiculated. Staining for FN was also minimal over the collagen in normal dermis and the deeper, larger collagen fascicles in the lesions. Fibroblasts cultured from normal dermis, hypertrophic scar, and keloid for 5-6 weeks were intensely stained for FN. Extracellular matrix was heavily positive in cultures from the lesions compared with those from normal dermis.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalCell and Tissue Research
Volume231
Issue number1
DOIs
StatePublished - May 1 1983

Keywords

  • Cell culture
  • Fibronectin
  • Hypertrophic scar
  • Immunofluorescence
  • Keloid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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