Flexible opto-electronics enabled microfluidics systems with cloud connectivity for point-of-care micronutrient analysis

Stephen Lee*, A. J. Aranyosi, Michelle D. Wong, Ji Hyung Hong, Jared Lowe, Carol Chan, David Garlock, Scott Shaw, Patrick D. Beattie, Zachary Kratochvil, Nick Kubasti, Kirsten Seagers, Roozbeh Ghaffari, Christina D. Swanson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In developing countries, the deployment of medical diagnostic technologies remains a challenge because of infrastructural limitations (e.g. refrigeration, electricity), and paucity of health professionals, distribution centers and transportation systems. Here we demonstrate the technical development and clinical testing of a novel electronics enabled microfluidic paper-based analytical device (EE-μPAD) for quantitative measurement of micronutrient concentrations in decentralized, resource-limited settings. The system performs immune-detection using paper-based microfluidics, instrumented with flexible electronics and optoelectronic sensors in a mechanically robust, ultrathin format comparable in size to a credit card. Autonomous self-calibration, plasma separation, flow monitoring, timing and data storage enable multiple devices to be run simultaneously. Measurements are wirelessly transferred to a mobile phone application that geo-tags the data and transmits it to a remote server for real time tracking of micronutrient deficiencies. Clinical tests of micronutrient levels from whole blood samples ( n=95) show comparable sensitivity and specificity to ELISA-based tests. These results demonstrate instantaneous acquisition and global aggregation of diagnostics data using a fully integrated point of care system that will enable rapid and distributed surveillance of disease prevalence and geographical progression.

Original languageEnglish (US)
Pages (from-to)290-299
Number of pages10
JournalBiosensors and Bioelectronics
Volume78
DOIs
StatePublished - Apr 15 2016

Keywords

  • Diagnostic
  • Flexible microelectronics
  • Micronutrients
  • Near field communication
  • Paper microfluidic assay
  • Point-of-care

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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