@article{663fafbb360f4a26a769f5317295b5e8,
title = "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex",
abstract = "To identify CAP3 and CAP4, components of the CD95 (Fas/APO-1) death- inducing signaling complex, we utilized nano-electrospray tandem mass spectrometry, a recently developed technique to sequence femtomole quantities of polyacrylamide gel-separated proteins. Interestingly, CAP4 encodes a novel 55 kDa protein, designated FLICE, which has homology to both FADD and the ICE/CED-3 family of cysteine proteases. FLICE binds to the death effector domain of FADD and upon overexpression induces apoptosis that is blocked by the ICE family inhibitors, CrmA and z-VAD-fmk. CAP3 was identified as the FLICE prodomain which likely remains bound to the receptor after proteolytic activation. Taken together, this is unique biochemical evidence to link a death receptor physically to the proapoptotic proteases of the ICE/CED-3 family.",
author = "Marta Muzio and Chinnaiyan, {Arul M.} and Kischkel, {Frank C.} and Karen O'Rourke and Andrej Shevchenko and Jian Ni and Carsten Scaffidi and Bretz, {James D.} and Mei Zhang and Reiner Gentz and Matthias Mann and Krammer, {Peter H.} and Peter, {Marcus E.} and Dixit, {Vishva M.}",
note = "Funding Information: M. M. is supported by an Associazione Italiana per la Ricerca su Cancro (AIRC) fellowship. A. M. C. is a fellow of the Medical Scientist Training Program supported by the Experimental Immunopathology Training Grant. The initial clone used in this study was discovered as part of a joint collaboration between the scientists at The Institute for Genomic Research and at Human Genome Sciences. We are especially grateful to Dr. Kim Orth and Dr. M. Wilm for helpful discussions and experimental support, Dr. Guy S. Salvesen for anti-GraB, Dr. Christopher J. Froelich for granzyme B, and Dr. Guy G. Poirier for purified PARP and anti-PARP antibody (C-2–10). We are grateful for the expert technical assistance of K. Krieger, U. Silberzahn, and Y. Kuang. We would like to thank Ian M. Jones for help in preparing the figures, Dennis Martin and the University of Michigan Oligo Core Facility, and Robert Lyons and the University of Michigan Sequencing Core Facility. Work at the Protein & Peptide Group is partially supported by a generous grant of the German Technology Ministry (BMBF). This work was supported by grants from BMBF (Bonn) and the Tumor Center (Heidelberg/Mannheim, Germany). ",
year = "1996",
month = jun,
day = "14",
doi = "10.1016/S0092-8674(00)81266-0",
language = "English (US)",
volume = "85",
pages = "817--827",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}