Flow cytometric identification of TFH13 cells in mouse and human

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

11 Scopus citations

Abstract

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper TFH13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying TFH13 cells in both mice and humans.

Original languageEnglish (US)
Pages (from-to)470-483
Number of pages14
JournalJournal of Allergy and Clinical Immunology
Volume147
Issue number2
DOIs
StatePublished - Feb 2021

Keywords

  • IL-13
  • IL-4
  • IL-5
  • IgE
  • T follicular helper cells
  • T13 cells
  • allergy
  • intracellular cytokine staining

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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