Flow cytometric methods for the analysis of human basophil surface antigens and viability

Bruce S. Bochner; Alicia A. McKelvey; Robert P. Schleimer; James E K Hildreth; Donald W. MacGlashan

doi:10.1016/0022-1759(89)90102-6

Flow cytometric methods for the analysis of human basophil surface antigens and viability

Bruce S. Bochner^*, Alicia A. McKelvey, Robert P. Schleimer, James E K Hildreth, Donald W. MacGlashan

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.

Original language	English (US)
Pages (from-to)	265-271
Number of pages	7
Journal	Journal of Immunological Methods
Volume	125
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(89)90102-6
State	Published - Dec 20 1989

Funding

Correspondence to: B.S. Bochner, The Johns Hopkins Asthma and Allergy Center, Clinical Immunology Unit Office 3, 301 Bayview Blvd., Baltimore, MD 21224, U.S.A. * Dr. Bochner was supported in part by a New Investigator Award from the American Lung Association; this work was also supported by National Institutes of Health Grants AM31891 and AI20136. Publication no. 768 from the O'Neill Research Laboratories at the Good Samaritan Hospital.

Keywords

Basophil
Flow cytometry
IgE
Immunofluorescence
Viability

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(89)90102-6

Cite this

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title = "Flow cytometric methods for the analysis of human basophil surface antigens and viability",

abstract = "Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.",

keywords = "Basophil, Flow cytometry, IgE, Immunofluorescence, Viability",

author = "Bochner, {Bruce S.} and McKelvey, {Alicia A.} and Schleimer, {Robert P.} and Hildreth, {James E K} and MacGlashan, {Donald W.}",

note = "Funding Information: Correspondence to: B.S. Bochner, The Johns Hopkins Asthma and Allergy Center, Clinical Immunology Unit Office 3, 301 Bayview Blvd., Baltimore, MD 21224, U.S.A. * Dr. Bochner was supported in part by a New Investigator Award from the American Lung Association; this work was also supported by National Institutes of Health Grants AM31891 and AI20136. Publication no. 768 from the O'Neill Research Laboratories at the Good Samaritan Hospital.",

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doi = "10.1016/0022-1759(89)90102-6",

language = "English (US)",

volume = "125",

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journal = "Journal of Immunological Methods",

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AU - Bochner, Bruce S.

AU - McKelvey, Alicia A.

AU - Schleimer, Robert P.

AU - Hildreth, James E K

AU - MacGlashan, Donald W.

N1 - Funding Information: Correspondence to: B.S. Bochner, The Johns Hopkins Asthma and Allergy Center, Clinical Immunology Unit Office 3, 301 Bayview Blvd., Baltimore, MD 21224, U.S.A. * Dr. Bochner was supported in part by a New Investigator Award from the American Lung Association; this work was also supported by National Institutes of Health Grants AM31891 and AI20136. Publication no. 768 from the O'Neill Research Laboratories at the Good Samaritan Hospital.

PY - 1989/12/20

Y1 - 1989/12/20

N2 - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.

AB - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.

KW - Basophil

KW - Flow cytometry

KW - IgE

KW - Immunofluorescence

KW - Viability

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Flow cytometric methods for the analysis of human basophil surface antigens and viability

Abstract

Funding

Keywords

ASJC Scopus subject areas

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