Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

Hammad A. Faizi, Cody J. Reeves, Vasil N. Georgiev, Petia M. Vlahovska*, Rumiana Dimova

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

Original languageEnglish (US)
Pages (from-to)8996-9001
Number of pages6
JournalSoft Matter
Volume16
Issue number39
DOIs
StatePublished - Oct 21 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

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