Abstract
A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8996-9001 |
| Number of pages | 6 |
| Journal | Soft Matter |
| Volume | 16 |
| Issue number | 39 |
| DOIs | |
| State | Published - Oct 21 2020 |
Funding
This research was funded in part by NSF-CMMI awards 1748049 and 1740011. PV acknowledges support from the Alexander von Humboldt Foundation and HAF acknowledges financial support from Prof. Reinhard Lipowsky for visits to the Max Planck Institute of Colloids and Interfaces. VNG and RD acknowledge support of the MaxSynBio consortium which is jointly funded by the Federal Ministry of Education and Research of Germany and the Max Planck Society. We thank Paul Salipante for proofreading the manuscript and M. Turner for useful suggestions. Open Access funding provided by the Max Planck Society.
ASJC Scopus subject areas
- General Chemistry
- Condensed Matter Physics