Fluid mechanics of electroosmotic flow and its effect on band broadening in capillary electrophoresis

Sandip Ghosal*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

149 Scopus citations

Abstract

Electroosmotic flow (EOF) usually accompanies electrophoretic migration of charged species in capillary electrophoresis unless special precautions are taken to supress it. The presence of the EOF provides certain advantages in separations. It is an alternative to mechanical pumps, which are inefficient and difficult to build at small scales, for transporting reagents and analytes on microfluidic chips. The downside is that any imperfection that distorts the EOF profile reduces the separation efficiency. In this paper, the basic facts about EOF are reviewed from the perspective of fluid mechanics and its effect on separations in free solution capillary zone electrophoresis is discussed in the light of recent advances.

Original languageEnglish (US)
Pages (from-to)214-228
Number of pages15
JournalELECTROPHORESIS
Volume25
Issue number2
DOIs
StatePublished - Jan 2004

Keywords

  • Band broadening
  • Capillary electrophoresis
  • Electroosmosis
  • Lubrication theory
  • Review
  • Taylor dispersion

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'Fluid mechanics of electroosmotic flow and its effect on band broadening in capillary electrophoresis'. Together they form a unique fingerprint.

Cite this