Fluorescence detection of tumors: studies on the early diagnosis of microscopic lesions in preclinical and clinical studies (Invited Paper)

Thomas S. Mang*, Carolyn McGinnis, David H. Crean, S. Khan, Charles D M D Liebow

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

The growth of microscopic tumor lesions at or beyond treatment field lesions poses major problems in the diagnosis and curative treatment of numerous cancers. Early detection techniques which clearly define the extent of condemned or field spread of disease may improve the primary treatment of the disease. In vivo fluorescence photometry is a non-imaging technique which digitally displays relative fluorescence values in volts. The sensitivity of the instrument has allowed the detection of micrometastases in both pre-clinical and clinical studies using drug doses that are 80-90% lower than those used therapeutically. This technique is now being applied in preliminary experiments to the hamster cheek pouch models to (1) discern varying grades of dysplasia; (2) levels of uptake of the drug in normal growing and quiescent tumors. Results will be shown in two models in which this technique has shown to be efficacious preclinically in the Pollard rat adenocarcinoma model in which micrometastases in the lymph node have been detected, and preliminary studies involving the hamster cheek pouch model in which the pouch is painted with 9, 10 dimethyl-1, 2-benzanthracene (DMBA) for initiation and promotion of tumors. Clinically results will be shown in which fluorescence detection, confirmed by biopsy and histopathological examination, was capable of detecting the existence of micrometastatic involvement of less than 100 cells.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages97-110
Number of pages14
Volume1426
ISBN (Print)0819405167
StatePublished - Jan 1 1991
EventOptical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques - Los Angeles, CA, USA
Duration: Jan 23 1991Jan 25 1991

Other

OtherOptical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques
CityLos Angeles, CA, USA
Period1/23/911/25/91

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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