TY - JOUR
T1 - Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
AU - Mogharbel, Bassam Felipe
AU - Francisco, Julio Cesar
AU - Irioda, Ana Carolina
AU - Dziedzic, Dilcele Silva Moreira
AU - Ferreira, Priscila Elias
AU - de Souza, Daiany
AU - de Souza, Carolina Maria Costa Oliveira
AU - Neto, Nelson Bergonse
AU - Guarita-Souza, Luiz Cesar
AU - Franco, Celia Regina Cavichiolo
AU - Nakamura, Celso Vataru
AU - Kaplum, Vanessa
AU - Mazzarino, Letícia
AU - Lemos-Senna, Elenara
AU - Borsali, Redouane
AU - Soto, Paula A.
AU - Setton-Avruj, Patricia
AU - Abdelwahid, Eltyeb
AU - de Carvalho, Katherine Athayde Teixeira
N1 - Funding Information:
We would like to thank financial support of Institut Carnot POLYNAT (France), CERMAV (France), Luciana Lopes from Instituto de Tecnologia do Paraná (TECPAR) (Brazil) for the Vero cell line supply, and Coordenação de Aperfeiçoa-mento de Pessoal de Nível Superior (CAPES) (Brazil).
Publisher Copyright:
© 2018 Mogharbel et al.
PY - 2018
Y1 - 2018
N2 - Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.
AB - Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.
KW - Cell marking
KW - Mesenchymal stem cells
KW - Myocardium infarction
KW - Sciatic nerve crush
KW - Transplantation
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U2 - 10.2147/IJN.S171099
DO - 10.2147/IJN.S171099
M3 - Article
C2 - 30319253
AN - SCOPUS:85054891256
SN - 1176-9114
VL - 13
SP - 5823
EP - 5836
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
ER -