Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

Bassam Felipe Mogharbel; Julio Cesar Francisco; Ana Carolina Irioda; Dilcele Silva Moreira Dziedzic; Priscila Elias Ferreira; Daiany de Souza; Carolina Maria Costa Oliveira de Souza; Nelson Bergonse Neto; Luiz Cesar Guarita-Souza; Celia Regina Cavichiolo Franco; Celso Vataru Nakamura; Vanessa Kaplum; Letícia Mazzarino; Elenara Lemos-Senna; Redouane Borsali; Paula A. Soto; Patricia Setton-Avruj; Eltyeb Abdelwahid; Katherine Athayde Teixeira de Carvalho

doi:10.2147/IJN.S171099

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

Bassam Felipe Mogharbel, Julio Cesar Francisco, Ana Carolina Irioda, Dilcele Silva Moreira Dziedzic, Priscila Elias Ferreira, Daiany de Souza, Carolina Maria Costa Oliveira de Souza, Nelson Bergonse Neto, Luiz Cesar Guarita-Souza, Celia Regina Cavichiolo Franco, Celso Vataru Nakamura, Vanessa Kaplum, Letícia Mazzarino, Elenara Lemos-Senna, Redouane Borsali, Paula A. Soto, Patricia Setton-Avruj, Eltyeb Abdelwahid, Katherine Athayde Teixeira de Carvalho

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.

Original language	English (US)
Pages (from-to)	5823-5836
Number of pages	14
Journal	International Journal of Nanomedicine
Volume	13
DOIs	https://doi.org/10.2147/IJN.S171099
State	Published - 2018

Funding

We would like to thank financial support of Institut Carnot POLYNAT (France), CERMAV (France), Luciana Lopes from Instituto de Tecnologia do Paraná (TECPAR) (Brazil) for the Vero cell line supply, and Coordenação de Aperfeiçoa-mento de Pessoal de Nível Superior (CAPES) (Brazil).

Keywords

Cell marking
Mesenchymal stem cells
Myocardium infarction
Sciatic nerve crush
Transplantation

ASJC Scopus subject areas

Drug Discovery
Bioengineering
Biophysics
Biomaterials
Pharmaceutical Science
Organic Chemistry

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10.2147/IJN.S171099

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Mogharbel, B. F., Francisco, J. C., Irioda, A. C., Dziedzic, D. S. M., Ferreira, P. E., de Souza, D., de Souza, C. M. C. O., Neto, N. B., Guarita-Souza, L. C., Franco, C. R. C., Nakamura, C. V., Kaplum, V., Mazzarino, L., Lemos-Senna, E., Borsali, R., Soto, P. A., Setton-Avruj, P., Abdelwahid, E., & de Carvalho, K. A. T. (2018). Fluorescence properties of curcumin-loaded nanoparticles for cell tracking. International Journal of Nanomedicine, 13, 5823-5836. https://doi.org/10.2147/IJN.S171099

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title = "Fluorescence properties of curcumin-loaded nanoparticles for cell tracking",

abstract = "Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.",

keywords = "Cell marking, Mesenchymal stem cells, Myocardium infarction, Sciatic nerve crush, Transplantation",

author = "Mogharbel, {Bassam Felipe} and Francisco, {Julio Cesar} and Irioda, {Ana Carolina} and Dziedzic, {Dilcele Silva Moreira} and Ferreira, {Priscila Elias} and {de Souza}, Daiany and {de Souza}, {Carolina Maria Costa Oliveira} and Neto, {Nelson Bergonse} and Guarita-Souza, {Luiz Cesar} and Franco, {Celia Regina Cavichiolo} and Nakamura, {Celso Vataru} and Vanessa Kaplum and Let{\'i}cia Mazzarino and Elenara Lemos-Senna and Redouane Borsali and Soto, {Paula A.} and Patricia Setton-Avruj and Eltyeb Abdelwahid and {de Carvalho}, {Katherine Athayde Teixeira}",

note = "Publisher Copyright: {\textcopyright} 2018 Mogharbel et al.",

year = "2018",

doi = "10.2147/IJN.S171099",

language = "English (US)",

volume = "13",

pages = "5823--5836",

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Mogharbel, BF, Francisco, JC, Irioda, AC, Dziedzic, DSM, Ferreira, PE, de Souza, D, de Souza, CMCO, Neto, NB, Guarita-Souza, LC, Franco, CRC, Nakamura, CV, Kaplum, V, Mazzarino, L, Lemos-Senna, E, Borsali, R, Soto, PA, Setton-Avruj, P, Abdelwahid, E & de Carvalho, KAT 2018, 'Fluorescence properties of curcumin-loaded nanoparticles for cell tracking', International Journal of Nanomedicine, vol. 13, pp. 5823-5836. https://doi.org/10.2147/IJN.S171099

TY - JOUR

T1 - Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

AU - Mogharbel, Bassam Felipe

AU - Francisco, Julio Cesar

AU - Irioda, Ana Carolina

AU - Dziedzic, Dilcele Silva Moreira

AU - Ferreira, Priscila Elias

AU - de Souza, Daiany

AU - de Souza, Carolina Maria Costa Oliveira

AU - Neto, Nelson Bergonse

AU - Guarita-Souza, Luiz Cesar

AU - Franco, Celia Regina Cavichiolo

AU - Nakamura, Celso Vataru

AU - Kaplum, Vanessa

AU - Mazzarino, Letícia

AU - Lemos-Senna, Elenara

AU - Borsali, Redouane

AU - Soto, Paula A.

AU - Setton-Avruj, Patricia

AU - Abdelwahid, Eltyeb

AU - de Carvalho, Katherine Athayde Teixeira

PY - 2018

Y1 - 2018

N2 - Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.

AB - Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.

KW - Cell marking

KW - Mesenchymal stem cells

KW - Myocardium infarction

KW - Sciatic nerve crush

KW - Transplantation

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DO - 10.2147/IJN.S171099

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SN - 1176-9114

VL - 13

SP - 5823

EP - 5836

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

ER -

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

Abstract

Funding

Keywords

ASJC Scopus subject areas

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