Fluorescent droplet cytometry for on-cell phenotype tracking

Xiaolong Yang, Wenhan Liu, Darren Chi Hang Chan, Sharif U. Ahmed, Hansen Wang, Zongjie Wang, Carine R. Nemr, Shana O. Kelley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Profiling the heterogeneous phenotypes of live cancer cells is a key capability that requires single-cell analysis. However, acquiring information at the single-cell level for live cancer cells is challenging when small collections of cells are being targeted. Here, we report single-cell analysis for low abundance cells enabled by fluorescent droplet cytometry (FDC), an approach that uses a biomarker-specific enzymatic fluorescent assay carried out using a droplet microfluidic platform. FDC utilizes DNA-functionalized antibodies in droplets to achieve specific on-cell target detection and enables characterization and profiling of live cancer cells with single-cell resolution based on their surface phenotype. Using this approach, we achieve live-cell phenotypic profiling of multiple surface markers acquired with small (<40 cells) collections of cells.

Original languageEnglish (US)
Pages (from-to)14805-14809
Number of pages5
JournalJournal of the American Chemical Society
Volume142
Issue number35
DOIs
StatePublished - Sep 2 2020
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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