Follicle microenvironment-associated alterations in gene expression in the mouse oocyte and its polar body

Ze Xu Jiao, Teresa K. Woodruff*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Objective: To determine whether the follicle environment modulates oocyte-specific gene transcript levels in cultured oocytes and polar bodies (PBs). Design: Animal study. Setting: Large academic research center. Animal(s): CD1 mice. Intervention(s): In vitro growth of secondary mouse follicles in 0.25% or 1.5% alginate (ALG) in a three-dimensional culture system. Main Outcome Measure(s): Relative transcript levels of Gdf9, Bmp15, Nlrp5, Tcl1, and Zp3 were measured by real-time quantitative reverse transcriptase-polymerase chain reaction in oocytes during in vitro follicle development and oocyte maturation and in their first PBs after removal from metaphase II (MII) eggs. Result(s): All transcripts decreased earlier in oocytes cultured in 1.5% ALG compared with 0.25% ALG. Transcript levels were lower in MII eggs cultured in 1.5% ALG compared with in 0.25% ALG. All genes were expressed in PBs, and transcript levels were lower in PBs cultured in 1.5% ALG compared with in 0.25% ALG. Abundance of all transcripts was lower in PBs than in their sibling oocytes. Conclusion(s): Local follicle environment modulates oocyte-specific gene expression in the oocyte and first PB. There is a significant difference in the transcript levels of oocyte-specific genes in PBs of 1.5% versus 0.25% ALG that correlates with ovarian environment-related decreases in oocyte competence.

Original languageEnglish (US)
Pages (from-to)1453-1459e1
JournalFertility and Sterility
Volume99
Issue number5
DOIs
StatePublished - Apr 2013

Keywords

  • Infertility
  • follicle culture
  • meiosis
  • oocyte quality
  • oocyte-specific genes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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