TY - JOUR
T1 - Fontimonas thermophila gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a freshwater hot spring, and proposal of Solimonadaceae fam. nov. to replace Sinobacteraceae Zhou et al. 2008
AU - Losey, Nathaniel A.
AU - Stevenson, Bradley S.
AU - Verbarg, Susanne
AU - Rudd, Stephen
AU - Moore, Edward R.B.
AU - Lawson, Paul A.
PY - 2013
Y1 - 2013
N2 - A novel bacterial strain designated HA-01T was isolated from a freshwater terrestrial hot spring located at Hot Springs National Park, Arkansas, USA. Cells were Gram-negative-staining, rodshaped, aerobic, chemo-organotrophic, oxidaseand catalase-positive, non-spore-forming and motile by means of a single polar flagellum. Growth occurred at 37-60 °C, with an optimum between 45 and 50 6°, and at pH 6.5-8.5, with an optimum between pH 6.5 and 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of strain HA-01T were Solimonas aquatica NAA-16T (93.8%), Solimonas flava CW-KD 4T (94.1%), Solimonas soli DCY12T (93.1%), Solimonas variicoloris MN28T (94.0%), Nevskia ramosa Soe1T (91.2%) and Hydrocarboniphaga effusa AP103T (91.1%). Major fatty acids consisted of C16: 0, iso-C16: 0, C16: 1ω5c and summed feature 8 (C18: 1ω9c, C18: 1ω7c and C18: 1ω6c). Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major isoprenoid quinone was Q-8. The DNA G+C content was 64.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain HA-01T represents a novel species in a new genus for which the name Fontimonas thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is HA-01T (5DSM 23609T5CCUG 59713T). A new family, Solimonadaceae fam. nov., is also proposed to replace Sinobacteriaceae Zhou et al. 2008.
AB - A novel bacterial strain designated HA-01T was isolated from a freshwater terrestrial hot spring located at Hot Springs National Park, Arkansas, USA. Cells were Gram-negative-staining, rodshaped, aerobic, chemo-organotrophic, oxidaseand catalase-positive, non-spore-forming and motile by means of a single polar flagellum. Growth occurred at 37-60 °C, with an optimum between 45 and 50 6°, and at pH 6.5-8.5, with an optimum between pH 6.5 and 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of strain HA-01T were Solimonas aquatica NAA-16T (93.8%), Solimonas flava CW-KD 4T (94.1%), Solimonas soli DCY12T (93.1%), Solimonas variicoloris MN28T (94.0%), Nevskia ramosa Soe1T (91.2%) and Hydrocarboniphaga effusa AP103T (91.1%). Major fatty acids consisted of C16: 0, iso-C16: 0, C16: 1ω5c and summed feature 8 (C18: 1ω9c, C18: 1ω7c and C18: 1ω6c). Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major isoprenoid quinone was Q-8. The DNA G+C content was 64.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain HA-01T represents a novel species in a new genus for which the name Fontimonas thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is HA-01T (5DSM 23609T5CCUG 59713T). A new family, Solimonadaceae fam. nov., is also proposed to replace Sinobacteriaceae Zhou et al. 2008.
UR - http://www.scopus.com/inward/record.url?scp=84872451683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872451683&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.037127-0
DO - 10.1099/ijs.0.037127-0
M3 - Article
C2 - 22407788
AN - SCOPUS:84872451683
VL - 63
SP - 254
EP - 259
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 1
ER -