Formation of smooth muscle α actin filaments in CD34+ bone marrow cells on arterial elastic laminae: Potential role of SH2 domain-containing protein tyrosine phosphatase-1

Shu Qian Liu*, Brandon J. Tefft, Andy Zhang, Li-Qun Zhang, Yu H. Wu

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs. Here, we investigated the role of elastic laminae in regulating the formation of SM α actin filaments in mouse CD34+ bone marrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process. Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 in mediating the formation of SM α actin filaments. In cell culture tests, elastic laminae, but not collagen matrix, stimulated the formation of SM α actin filaments in CD34+ cells. The phosphatase SHP-1 mediated the stimulatory effect of elastic laminae. The interaction of CD34+ cells with elastic laminae, but not with collagen matrix, induced activation of SHP-1. The suppression of SHP-1 by transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown significantly reduced the formation of SM α actin filaments in CD34+ cells cultured on elastic laminae. The in vitro observations were confirmed by using an in vivo model of implantation of elastic lamina and collagen matrix scaffolds into the aorta. These observations suggest that elastic laminae stimulate the formation of SM α actin filaments in CD34+ bone marrow cells and SHP-1 mediates the stimulatory effect of elastic laminae.

Original languageEnglish (US)
Pages (from-to)282-294
Number of pages13
JournalMatrix Biology
Volume27
Issue number4
DOIs
StatePublished - May 1 2008

Fingerprint

SH2 Domain-Containing Protein Tyrosine Phosphatases
Protein Phosphatase 1
Actin Cytoskeleton
Bone Marrow Cells
Smooth Muscle
Collagen
Small Interfering RNA
Smooth Muscle Myocytes
Non-Receptor Type 6 Protein Tyrosine Phosphatase
Protein Tyrosine Phosphatases
Cell Communication
Aorta
Cultured Cells
Cell Culture Techniques

Keywords

  • CD34+ bone marrow cells
  • Extracellular matrix
  • Protein tyrosine phosphatase SHP-1
  • Vascular smooth muscle cells

ASJC Scopus subject areas

  • Molecular Biology

Cite this

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title = "Formation of smooth muscle α actin filaments in CD34+ bone marrow cells on arterial elastic laminae: Potential role of SH2 domain-containing protein tyrosine phosphatase-1",
abstract = "Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs. Here, we investigated the role of elastic laminae in regulating the formation of SM α actin filaments in mouse CD34+ bone marrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process. Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 in mediating the formation of SM α actin filaments. In cell culture tests, elastic laminae, but not collagen matrix, stimulated the formation of SM α actin filaments in CD34+ cells. The phosphatase SHP-1 mediated the stimulatory effect of elastic laminae. The interaction of CD34+ cells with elastic laminae, but not with collagen matrix, induced activation of SHP-1. The suppression of SHP-1 by transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown significantly reduced the formation of SM α actin filaments in CD34+ cells cultured on elastic laminae. The in vitro observations were confirmed by using an in vivo model of implantation of elastic lamina and collagen matrix scaffolds into the aorta. These observations suggest that elastic laminae stimulate the formation of SM α actin filaments in CD34+ bone marrow cells and SHP-1 mediates the stimulatory effect of elastic laminae.",
keywords = "CD34+ bone marrow cells, Extracellular matrix, Protein tyrosine phosphatase SHP-1, Vascular smooth muscle cells",
author = "Liu, {Shu Qian} and Tefft, {Brandon J.} and Andy Zhang and Li-Qun Zhang and Wu, {Yu H.}",
year = "2008",
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Formation of smooth muscle α actin filaments in CD34+ bone marrow cells on arterial elastic laminae : Potential role of SH2 domain-containing protein tyrosine phosphatase-1. / Liu, Shu Qian; Tefft, Brandon J.; Zhang, Andy; Zhang, Li-Qun; Wu, Yu H.

In: Matrix Biology, Vol. 27, No. 4, 01.05.2008, p. 282-294.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Formation of smooth muscle α actin filaments in CD34+ bone marrow cells on arterial elastic laminae

T2 - Potential role of SH2 domain-containing protein tyrosine phosphatase-1

AU - Liu, Shu Qian

AU - Tefft, Brandon J.

AU - Zhang, Andy

AU - Zhang, Li-Qun

AU - Wu, Yu H.

PY - 2008/5/1

Y1 - 2008/5/1

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AB - Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs. Here, we investigated the role of elastic laminae in regulating the formation of SM α actin filaments in mouse CD34+ bone marrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process. Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 in mediating the formation of SM α actin filaments. In cell culture tests, elastic laminae, but not collagen matrix, stimulated the formation of SM α actin filaments in CD34+ cells. The phosphatase SHP-1 mediated the stimulatory effect of elastic laminae. The interaction of CD34+ cells with elastic laminae, but not with collagen matrix, induced activation of SHP-1. The suppression of SHP-1 by transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown significantly reduced the formation of SM α actin filaments in CD34+ cells cultured on elastic laminae. The in vitro observations were confirmed by using an in vivo model of implantation of elastic lamina and collagen matrix scaffolds into the aorta. These observations suggest that elastic laminae stimulate the formation of SM α actin filaments in CD34+ bone marrow cells and SHP-1 mediates the stimulatory effect of elastic laminae.

KW - CD34+ bone marrow cells

KW - Extracellular matrix

KW - Protein tyrosine phosphatase SHP-1

KW - Vascular smooth muscle cells

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